formation of aerosols should be used. Working surfaces should be 

 wiped down with an appropriate disinfectant before and after work 

 with each cell culture and at the end of the working day. 



3. Decontamination and Disposal 



Contaminated glassware and similar materials should be 

 appropriately decontaminated or stored for decontamination before 

 removal from the work area for recycling or disposal. Liquid 

 wastes should be decontaminated either chemically or by heat, 

 before being discharged to the community sanitary sewer system. 



4. Protection of Vacuum Lines 



Vacuum services, if used, should be protected with disposable 

 absolute air filters and liquid traps. The effluent should be 

 collected in liquid traps containing concentrated disinfectant. 



References 



Biohazards in Biological Research , ed. A. Hellman, M.N. Oxman and 

 P. Pollack. Cold Spring Harbor Laboratory, New York (1973). 



National Cancer Institute Specification: General Purpose Clean 

 Air Biological Safety Cabinet. 



15 



