committee and discussed with the research staff before starting 

 the research program. 



2. Medical Surveillance and Screening 



a. Physical Examinations 



Appropriate pre-employment and periodic medical examinations 

 are desirable for persons working with human cell lines. 



b. Work Restrictions 



Persons having reduced immunologic competency should be 

 restricted from working with these human cell lines. 



c. Serum Collection 



Serum should be collected at the time of the pre-employment 

 physical to establish a baseline reference. Serum should be re- 

 collected and stored annually. Serum should be collected immedi- 

 ately after accidental injection or ingestion and at an appropriate 

 interval thereafter. For those individuals exposed to long terra 

 lymphoid lines or their derivatives, anti-EB virus titers should be 

 obtained on the collected sera. 



3. Laboratory Access 



Access to the cell culture area should be restricted to 

 persons directly working with the cell lines, or by specific 

 authorization by the principle investigator or director of the 

 laboratory . 



B. Personnel Practices 

 1. Pipetting 



13 



