Mechanical pipetting aids rather than mouth pipetting should 

 be used for all pipetting procedures. 



2. Eating, Drinking and Smoking 



Eating, drinking and smoking should not be allowed in the same 

 areas where cell lines are under study. 



3. Protective Clothing 



It is recognized that the criteria for protective clothing may 

 vary according to the physical situation of the laboratory and the 

 agents handled. Ideally, adequate protective clothing such as a 

 fully fastened laboratory coat should be worn. This clothing 

 should not be worn outside the work area once the work area has 

 been entered. 



C. Physical Control Practices (Recommended for all cell lines, 

 but required for long term lymphoid lines and their deri- 

 atives) . 



1. Ventilated Safety Cabinets or Hoods 

 Ventilated safety cabinets and hoods and other safety 



apparatus should be employed and should be tested at least annually 

 to certify correct containment and operation. A list of speci- 

 fications for satisfactory hoods and instructional materials may be 

 obtained from the Office of Biohazards, NCI. 



2. Housekeeping 



Appropriate housekeeping procedures which suppress the 



14 



