6. HOW SHIPPED 



All cell cultures are grown and frozen in antibiotic-free 

 media to aid in detection and prevention of contamination. Cell 

 cultures in the Repository have been tested and found free of 

 mycoplasma, bacteria, molds and fungi during characterization, 

 at the time of frozen storage, and after recovery from liquid 

 nitrogen. When an order is received, a frozen ampule is usually 

 recovered from liquid nitrogen on the following Thursday and the 

 medium is changed on Friday. The following Monday, the culture 

 is inspected, the medium is removed and the T25 flask is filled 

 with fresh medium, packed and shipped, usually by air mail 

 special delivery. The flask is enclosed within two watertight 

 plastic envelopes within a styrofoam box in a cardboard mailer 

 to prevent leakage, overheating or freezing during shipment. 

 A return postcard is enclosed. Please return the postcard with 

 notations about the condition on arrival. Pertinent suggestions 

 to improve the effectiveness of the Repository are appreciated. 



7. DESCRIPTIVE DATA 



Each shipment contains descriptive data about the cell 

 culture, suggested directions for cell growth and pertinent 

 references when available. Upon receipt cell cultures should 

 be placed in the incubator at 37° C for a few hours or overnight 

 to permit recovery from damage which may have occurred during 

 shipment . 



