APPENDIX A 



CODE FOR CULTURE MEDIA 



For reference see: Morton, Helen C, A survey of Commercially 



Available Tissue Culture Media. In Vitro 6:89-108, 1970. 



A - McCoy's 5A with 20% fetal bovine serum (FBS) not inactivated 

 (Iwakata and Grace modification) 



B - Ham F12 with 20% FBS not inactivated 



C - Minimum Essential Medium Eagle in Earle's BSS with 20% FBS 

 not inactivated (with 2x concentration of essential and 

 non-essential amino acids and vitamins) 



D - Roswell Park Memorial Institute 1640 with 10% FBS inac- 

 tivated 60°C for 1/2 hour 



E - Roswell Park Memorial Institute 1640 with 20% FBS inactivated 

 60° C for 1/2 hour 



F - Minimum Essential Medium Eagle in Earle's BSS with 30% FBS 



not inactivated (with 2x concentration of essential and non- 

 essential amino acids and vitamins) 



G - Ham F12 with 10% FBS not inactivated 



H - McCoy's 5A with 10% FBS not inactivated (Iwakata and Grace 

 modification) 



I - Roswell Park Memorial Institute 1640 with 20% FBS not inac- 

 tivated 



J - Minimum Essential Medium Eagle in Earle's BSS with 10% FBS 

 not inactivated (with 2x concentration of esssential and 

 non-essential amino acids and vitamins) 



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