Abstracts: 

 Physical Mapping 



Center for Human Genome Studies, Los Alamos 

 National Laboratory 



Robert K. Moyzis, C. E. Hildebrand. R. L. Stallings, and N. A. Doggett 



Lite Sciences Division. Los Alamos National Laboratory. Los Alamos. NM 87545 



(505) 667-3912. FTS 843-3912 



The Los Alamos Center tor Human Genome Studies will provide coordination, 

 technical oversight, and direction for the following interdisciplinary elements of the 

 Human Genome Program at Los Alamos: physical mapping, new technology 

 development, and infomiatics. The center will also develop collaborative research and 

 development programs with the private sector and with other centers for human genome 

 research. 



The goals of this project are ( 1 ) to develop concepts and to advance technology for 

 genomic physical mapping and (2) to construct a physical map of human chromosome 

 16 that will include an ordered set of overlapping DNA fragments encompassing the 

 chromosome. The physical map will integrate phage, cosmid, and yeast artificial 

 chromosome (YAC) contigs ordered by repetitive sequence "fingerprinting" with the 

 genetic linkage map. identified gene sequences, and the cytogenetic map into a tool that 

 will allow rapid access to any region of chromosome 16 for analysis and eventual large- 

 scale sequencing. The significance of this work lies in the immediate application of the 

 knowledge and tools (1 ) to understand human genetic disease. (2) to clarify the 

 molecular bases for genetic disease susceptibility, especially in regard to energy-related 

 chemical or radiation exposures, and (3) to reveal the molecular details underlying long- 

 range chromosome architecture and dynamics. 



72 



