Physical Mapping 



Physical map of contigs on chromosome 11. Researchers at The Salk 

 Institute and Yale University Medical School have generated a series of 

 overlapping sets of cosmids. or contigs, that vary from 2 to 27 clones. The 

 position and relative order of many of the contigs have been determined by using 

 fluorescence in situ hybridization on metaphase chromosome spreads, in 

 particular, the relative order of contigs containing known cloned genes, 

 anonymous DNA markers, or Hpa-ll-tiny-fragment (HTF) islands (possibly 

 indicating the location of as yet undescribed genes) are indicated for comparison 

 to the ideogram of chromosome 1 1 . The position of hybridization is determined by 

 the fractional length from the 11 p telomere (FLpter) rather than using cytogenetic 

 banding. Known genes that have been mapped on the long arm include those 

 encoding the neural cell adhesion molecule (NCAf\/l), the Thy-1 antigen, the 

 ApoAl cluster, the CDS cluster, porphyrinogen deaminase (PBG), the ETS1 

 oncogene, and the signal recognition particle receptor (SRPR), as well as others. 

 [Figure first published by the American Association for the Advancement of 

 Science in P. Lichter et al., "High-Resolution IVIapping of Human Chromosome 1 1 

 by in Situ Hybridization with Cosmid Clones," Science 247, 64-69 (Jan. 5, 1990). 

 Figure provided by Glen Evans, The Salk Institute, and Peter Lichter, Yale 

 University Medical School.] 



Fractional 

 length 



I J3-1 



I J2-2 



I 13~4A, 18-10B 



I PTH 

 I J4-17 



I HRAS 



I J7.1.HBBC 



I J5-3 



I J10-3. J2.2 

 I J10-17 

 I 4.4B 

 FSHB 



I 5-2A 

 I J1.2 



I piaL 



I PVGM 



I ZC7 I XB 

 I XBll 



I 9 27 , 



I I 66 



I 3 16 



I 23 20 



I NCAM 



, ,- , I '^^' I AP0AI.4 13,9 4. ZA7 



I ^'-^ I THYl I tD3D, PBGD. XB1. XH5 



111 I ZD8 I 



XBIO.ZDS. ZD7 



I SRPR 



I ETSI.23,2 



I XB2 



I 9« 

 8 5 



25 



