Abstracts: 

 Physical Mapping 



Correlation of Physical and Genetic Maps of Human 

 Chromosome 16 



Grant R. Sutherland. David F. Callen. Valentine J. Hyland, John C. Mulley, and 



Robert I. Richards 



Department of Histopathoiogy. Adelaide Children's Hospital, North Adelaide, 



South Australia 5006, Australia 



011-618-267-7333 



The goal of this project is to construct a detailed physical map, which will be correlated 

 with the linkage map, of human chromosome 16. The methods to be used for con- 

 struction include the following: 



(DA panel of mouse/human hybrid cell lines, which contain only parts of chromosome 

 16, will be developed. The panel will be achieved by fusing human cells that contain 

 rearrangements of chromosome 16 with mouse A9 cells and selecting for the human 

 APRT gene on the end of the long ami of chromosome 16. The cytogenetic and 

 molecular characterization of the panel will allow detailed physical mapping of cloned 

 DNA sequences and genes that are expressed in the hybrid cells. The cell panel 

 produced should divide this chromosome, which contains approximately 3.3% of the 

 human genome, into about 50 intervals of average-size 2 Mb and thus provide a means 

 of mapping any cloned DNA sequence from chromosome 16 into these relatively small 

 regions. Sequences that map into such a region should then be useful to generate 

 restriction maps using pulsed-field gel electrophoresis. This project should lay the 

 foundation for construction of a restriction map of chromosome 16. 



(2) Anonymous cloned DNA fragments of chromosome 16 will be selected from 

 various chromosome- 16-specific libraries and mapped using the hybrid cell panel. In 

 selected intervals these fragments will be used to identify restriction fragment length 

 polymorphisms (RFLPs) for which the CEPH (Centre d'Etude du Polymorphisme 

 Humain) panel of families will be typed to correlate the physical and linkage maps. 

 Probes to cloned genes that have been mapped to chromosome 16 will be obtained, and 

 these genes will be physically mapped: where these probes detect RFLPs, their linkage 

 relationships with other cloned segments will be determined using the CEPH families. 

 Probes on chromosome 16 that have been put through the CEPH families and used to 

 generate linkage maps will be obtained from other researchers on a collaborative basis 

 and physically mapped to further define the correlation of the physical and genetic maps 

 of this chromosome. 



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