New Approaches for Constructing Expression Maps 

 of Complex Genomes 



Sherman M. Weissman, R. Kandpal. A. Swaroop. S. Parimoo, H. Arenstorf. 



and D, Ward 



Department of Human Genetics, Yale University, New Haven, CT 06510 



(203)785-2677 



The overall objective of this project is to develop and demonstrate methods for 

 preparing normalized cDNA libraries and using them for gene mapping and mutation 

 detection. A phage vector has been prepared that can be used efficiently to generate 

 single-stranded cDNA clones. A number of human sources have been used to prepare 

 the cDNA libraries. Biotin avidin selection methods have been developed for con- 

 venient preparation of subtracted cDNA libraries and are currently being evaluated for 

 their ability to generate selected cDNA libraries containing only those cDNA 

 complementary to selected segments of the human genome. In addition, polymerase 

 chain reaction methodology is being adapted to make chromosome jumping a much 

 more efficient general procedure for long-range genome mapping and to provide 

 improved methods for preparing selected cDNA libraries. 



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