254 Transactions. 



of the fluid was concentrated and injected into a frog ; it caused death. 

 A similar quantity treated with alkali, neutralized, and injected into another 

 frog also caused death. Neither frog showed tutin symptoms. 



Foetal brains treated together in similar way and filtrate concentrated 

 to small hulk : whole filtrate hydrolyzed with HC1 gave slight but distinct 

 reduction. No test made before hydrolysis because of small bulk of fluid, 

 and result would need to be controlled by using normal foetal brains. 



Blood. — An extract of the blood of this animal gave reduction with 

 Fehling without hydrolyzing. To distinguish whether tutin was present, 

 the blood was evaporated to dryness on filter paper, and extracted with 

 hot ether ; the ethereal extract was extracted with water after driving off 

 the ether. This extract gave no reduction before or after treatment with 

 HC1. 



237. Young cat, weight about 850 grm., received orally the alcoholic 

 extract of dried blood of rabbit 234 (dosed with lOmlgrm. tutin). As 

 described in the text above, the extract represented about 33 c.c. of blood. 

 At 12 noon, dose administered ; 12.3 p.m., very distinct twitching of the ears ; 

 12.14 p.m., twitching continues, licking its jjps and swallowing frequently ; 

 12.22 p.m., drowsy appearance, deliberate winking, and exaggerated breath- 

 ing ; 1.3 p.m., no symptoms apparent except drowsiness. When seen again 

 at 3.18 p.m. it appeared normal. 



(If the tutin in rabbit 234 had been uniformly distributed, the 33 c.c. 

 of blood would have contained 0-164 mlgrm: this to a cat weighing 

 800 grm. = 0-2 mlgrm. per kilo. But a control' experiment on a sister cat 

 showed that 0-21 mlgrm. per kilo given orally in milk produced no effect. 

 We may conclude, therefore, that the blood of No. 234 contained more 

 than its due proportion of the poison.) 



238. Young cat, weight 760 grm., received an extract of the muscles 

 of rabbit 234 (dosed with 10 mlgrm. tutin). The dried muscle weighed 

 35 grm., representing, therefore, over 100 grm. body-flesh. The greater 

 part of this extract was successfully administered to the cat, and beyond 

 urination and some quickening of respiration no obvious effect followed. 



240. Rabbit, weight 1,350 grm., received 10 mlgrm. tutin intravenously. 

 Twenty-two minutes afterwards, when practically dead, the blood was 

 collected, and the liver, muscles, brain, and cord excised and treated in 

 the usual way for extraction of tutin. The extract of muscle, representing 

 100 grm. to 200 grm. of flesh produced no symptoms on oral administration 

 to a cat weighing 812 grm. The extract of liver, representing 30 grm. fresh 

 substance, tested on another young cat produced no symptoms beyond 

 urination and defaecation, which might be attributed to the bile salts and 

 other normal extractives. 



244. Young cat, weight 812 grm., received hypodermically 15 mlgrm. 

 tutin. Symptoms became severe in ten minutes, and lasted an hour, at 

 end of which time the animal was killed by bleeding. The temperature 

 fell steadily all the time (37° C. to 32° C. at death). A test-tubeful of blood 

 of this animal yielded very little serum, which did not reduce Fehling either 

 before or after hydrolyzing, while the blood of a normal cat killed by chloro- 

 forming at the same time gave the usual proportion of serum, and this 

 serum reduced Fehling easily before, and still more so after, hvdrolvzing 

 with HC1. 



My sincere thanks are due to Mr. Aston, of the Agricultural Depart- 

 ment, Wellington, for supplying me with pure crystallized tutin. 



