(+) 



• ••— 







Fig. 3, 



Zymogram demonstrating the similarities In LDH iso- 

 zyme pattern of heart tissues from 4 different species: 

 the mouse, cow, rabbit and chicken. Heart muscle LDH 

 from most vertebrate classes consists mainly of the more 

 anodally migrating Isozymes, LDH-1 and -2. 



Fig. 2. 



Zymogram of the Isozyme patterns of mixed tissue homo- 

 genates from 10 mammalian species. Note the differences 

 In electrophoretic mobility of homologous Isozymes 

 among the different species. LDH-1 is the fastest or 

 most anodally migrating (most negatively charged) band. 

 The slower moving bands, possessing a progressively 

 decreasing negative charge, are designated LDH- 2, 

 LDH-3, LDH-4, LDH-5, respectively. In our electro- 

 phoretic system, LDH-5 is essentially neutral and its 

 apparent cathodal movement is the resultant of electro- 

 endosmosis within the electrophoretic medium (starch 

 gel). (From Markert, in The Harvey Lectures, Series 59, 

 187, 1965; reproduced with permission of Academic 

 Press.) 



which is capable of indefinitely sustained 

 ("heart- like") activity, such as the breast 

 muscles of certain birds (8), and, as we have 

 recently observed, the flight muscles of bats (9), 

 contains a predominance of rapidly migrating 

 LDH isozymes. The similarities in LDH isozyme 

 patterns among homologous tissues are em- 

 phasized by the differences in the isozyme pat- 

 terns among heterologous tissues. This is 



Fig. 4. 



Zymogram of the LDH patterns of representative tissues 

 of the pike (left) and the whiting (right). Both fish are 

 members of the order Ostelchthys. Note the complex pat- 

 tern of Isozymes found in heart tissues of the whiting. 



79 



