60 120 180 240 300 360 



60 120 180 240 300 360 

 ml effluent 



Fig. 3. 



(A) Fractionation of soluble proteins from adult bovine lens 

 epithelial cells. The cells were homogenized in 0.005 HI 

 phosphate buffer pH 7.0 and the homogenate was cleared 

 by centrifuging at 10,000 x g for lOmin. The supernatant 

 was dialyzed against 0.005 \! phosphate buffer overnight. 

 74.0 mg of protein were added to 10 g of DEAE-cellu- 

 lose; 60.29 mg protein were recovered at the end of the 

 experiment. Buffers were added to the column in the 

 following sequence: I. 50 ml 0.005 -1/ sodium-phosphate 

 pH 7; II. 50 ml 0.0075 M sodium-phosphate pH 6.5; III. 50 

 ml 0.01 M sodium-phosphate pH 6; IV. 75 ml 0.02 M so- 

 dium-phosphate pH 5.7; V. 50 ml 0.02 M sodium-phos- 

 phate pH 5.7+ 0.1 M NaCl; VI. 50 ml 0.1 ,V sodium- 

 phosphate pH 5.7 + 0.1 ,M NaCl; VII. 50 ml 0.1 M 

 sodium-phosphate pH 5.7 + 0.3 A/ NaCl. The fractions 

 were collected in 3 ml allquots. (B) Fractionation of 

 soluble proteins from cortex fibers of the adult bovine 

 lens. The elution sequence is the same as that shown above. 

 74.0 mg protein were placed on 10 g of DEAE-cellulose; 

 65.39 mg protein were recovered at the end of the experi- 

 ment. (C) Fractionation of soluble proteins from nucleus 

 fibers of adult bovine lens. The elution sequence is the 

 same as that shown above. 73.92 mg protein were placed 

 on 10 g of DEAE-cellulose; 43.77 mg protein were re- 

 covered at the end of the experiment. (Fig. 1, J. Papacon- 

 stantlnou, Biochim. Biophys. AcialOT, 81, 1965; reproduced 

 with permission of Elsevier Publishing Company.) 



10- 

 08- 

 06- 

 04- 



02 



\k±^ 



i-t—r^ 



60 120 ISO 210 300 360 



60 120 180 240 300 360 



60 120 ISO 240 300 360 

 ml effluent 



Fig. 4. 



<A) Fractionation of soluble proteins from epithelial cells 

 of calf lenses. 48.24 mg protein were placed on 10 g of 

 DEAE-cellulose; 41.63 mg protein were recovered at the 

 end of the experiment. Buffers were added to the column 

 in the sequence described in Fig. 3. (B) Fractionation of 

 soluble proteins from calf cortex fibers. 49.80 mg pro- 

 tein were placed on 10 g DEAE-cellulose; 51.40 mg pro- 

 tein were recovered at the end of the experiment. The 

 elution sequence is the same as that described above. 

 (C) Fractionation of soluble proteins from calf nucleus 

 fibers. 50.23 mg protein were placed on 10 g of DEAE- 

 cellulose; 49.90 mg were recovered at the end of the 

 experiment. The elution sequence is the same as that 

 described above. (Fig. 2, J. Papaconstantinou, Biochim. 

 Biophys. Ada 107, 81, 1965; reproduced with permission 

 of Elsevier Publishing Company.) 



51 



