Primary Isolation Using Guinea Pigs and Embryonated Eggs 



Tissue Specimen Obtained 



4- 

 I 



Laboratory Service 

 Immediately Available 



If Yes 



If No 



I 



Store tissue at 

 -70° C until . . 



4- 



4- 



ROUTINE 



DIAGNOSTIC 



LABORATORY 



Place tissue specimens in 

 10% Formalin for 

 subsequent use in: 



Prepare 10% tissue suspension 

 in PBS for subsequent use in: 



Pathology 

 studies 



Direct FA 

 tests 



Direct FA 

 tests 



Culture 

 attempts 



±. 



in vivo/in vitro 

 isolation attempts 



4- 



CAN BE REFERRED TO 

 APPROPRIATE REFERENCE 

 LABORATORY, AS NECESSARY 



Figure 1. Treatment of Tissue Specimens for Isolation of the Legionnaires' Disease Bacterium 



