Detection of Legionnaires' Disease Bacteria mi Clinical Specimens liy Direct Immunonuorescence 



Legends for Figures 



Figure 1. Smear of 48-h culture of LDB stained witli a 1:80 dilution of hotnologous cotijugate. Culture was 

 grown on modified Mueller-Hinton medium and suspended in phosphate buffered saline containing l^c Formalin. 

 Note that these cells resemble those seen in tissue. Photographed with a lOOX oil objective on 135-mm Ekta- 

 chrome 200 film. Magnification .X400 as photographed. 



Figure 2. Smear of 5-day-old culture of LDB grown on a different modification of Mueller-Hinton medium. 

 Stained and photographed as in Figure 1. 



Figure 3. Deparaffinized section of LDB-infected human lung tissue showing positive FA staining of serogroup 2 

 LDB with serogroup 2 conjugate. The Atlanta 1 isolate (serogroup 2) was obtained from a fresh-frozen specimen 

 of the same lung tissue. Photographed with 25X objective. 



Figure 4. Deparaffinized section of lung tissue showing negative FA staining with serogroup 1 conjugate on tissue 

 infected with serogroup 2 LDB. The lung tissue was from the same paraffin-embedded block as that used in 

 Figure 3. Photographed with 40X objective. 



Figure 5. Deparaffinized section of heavily infected human lung tissue showing positive FA staining of LDB with 

 serogroup 3 conjugate. The tissue was FA negative when stained with conjugates for serogroups 1,2, and 4. 

 Photographed with 40X objective. 



Figure 6. LDB as they appear in a smear of scrapings of wet Formalin-fi.xed human lung tissue. Note large number 

 of extracellular bacteria, many of which have been released from broken tissue cells. Note absence of chains or 

 filamentous forms. Stained with a 1:80 dilution of homologous conjugate. Photographed as in Figure 1. 



Figure 7. LDB in sputum from a patient with LD. Stained with serogroup 1 conjugate. Photographed with lOOX 

 oil objective on 135-mm Ektachrome 400 film. Magnification X400 as photographed. 



Figure 8. LDB in sputum from a patient with LD. Stained and photographed as in Figure 7. Note filamentous 



forms. 



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