PART THREE 

 Methodology 



Primary Isolation Using Guinea Pigs and Embryonated Eggs 69 



Joseph E. McDade 



Primary Isolation Media and Methods 77 



James C. Feeley. George W. Gorman, and Robert J. Gibson 



Method for Isolating Legionnaires' Disease Bacterium from Soil and 



Water Samples 85 



George K. Morris. Peter Skaliy. Charlotte M. Patton. and Janies C. Feeley 



Detection of Legionnaires' Disease Bacteria in Clinical Specimens by 



Direct Immunofluorescence 91 



William B. Cherry and Roger M. Mc Kinney 



Demonstration of the Bacterium in Tissue by a Modification of the Dieterle 



Silver Impregnation Stain 105 



A.E. Van Orden and P.W. Greer 



Indirect Immunofluorescence Test for Legionnaires' Disease Ill 



Hazel W. Wilkinson. Donna D. Cruce. Bonnie J. Fikes. Lynn P. Yealy, 

 and Carol E. Farshy 



Analysis of Cellular Fatty Acids of Bacteria by Gas-Liquid Chromatography 117 



C Wayne Moss 



ELISA for Legionnaires' Disease - Antibody and Antigen Detection Systems: 



An Interim Report 123 



Carol E. Farshy cvid John C. Feeley 



PART FOUR 

 Policies, Resources and Services 



I. Laboratory Safety: Precautions and Practices 134 



II. Laboratory Services 



A. Pohcy 137 



B. Reference Material 137 



C. Reference Diagnostic Services 142 



D. Submitting Reference Specimens 144 



III. Epidemiologic Services 1 49 



A. Policy 149 



B. Epidemiologic Consultation and Assistance 149 



C. Clinical Consultation 149 



xi 



