Detection of Legionnaires' Disease Bacteria in Clinical Specimens by Direct Immunofluorescence 



II. LIST OF LDB ISOLATES 



A. Serogroup 1 isolates of the Knoxville 1 type include: 

 Knoxville 1 Pontiac 1 (E)* 

 Philadelpiiia 1-4 Miami Beach 1 

 Flint 1 and 2 Orlando 1 



Detroit 1-4 Indianapolis 1 and 2 



Beri<eley 1 Oak Lawn 1 



Bimiinghani 1 Baltimore 1 



Burlington 1 and 2 Houston 1 



Buffalo 1 Chicago 1 



Allentown 1 Long Beach 1 and 2 



Albuquerque 1 Cambridge 1 (England) 



Los Angeles 2-14 Atlanta 3 



New York 1 and 2 Bellingliam 1 



Olda Gastonia 1 



Rochester 1 TOTAL = 50 



B. Serogroup 2 isolates obtained to date include: 

 Togus 1 



Atlanta 1 and 2 



C. Serogroup 3 isolates obtained to date include the environmental representative Bloom- 

 ington 2 (E). The human isolates Chicago 2 and 3 and Houston 2 also stain with 

 serogroup 3 conjugate. 



D. Serogroup 4 is represented by the Los Angeles 1 strain obtained from human tissue. 



All strains listed above were obtained from human patients with LD except Blooming- 

 ton 2, an environmental isolate which is the type strain of serogroup 3, and Pontiac 1, which 

 was isolated from a guinea pig naturally infected by the aerosol route. Thus 49 or 87. 59^ of 

 the 56 liuman isolates listed above belong to serogroup 1 . A number of other environmental 

 isolates not included in the above list have been obtained that belong to serogroups 1-4. Still 

 other environmental isolates have been found that apparently represent new serogroups but 

 these cultures have not been completely characterized. 



REFERENCES 



1. Broome, C.V., W.B. Cheny, W.C. Winn, and B.R. MacPherson, Jr. IQV). Rapid diagnosis of Legionnaires' 

 disease by direct immunofluorescent staining. Ann. Intern. Med. 90:1-4. 



2. Center for Disease Control. 1978. Isolates of organisms resembling Legionnaires' disease bacterium from 

 environmental sources - Bloomington, Indiana. Morbid. Mortal. Weekly Rept. 27:283-285. 



3. Cherry, W.B., P.P. Harris, G.A. Hebert, B.M. Thomason, and R.M. McKinney. 1978. Legionnaires' disease - 

 the disease and the bacterium with special reference to the laboratory diagnosis. In Immunofluorescence and 

 Related Staining Techniques, W. Knapp, K. Ilolubar, and G. Wick (eds.). Elsevier/North-Holland Biomedical 

 Press, Amsterdam. 



4. Cherry, W.B., B. Pittman, P.P. Harris, G.A. Hebert. B.M. Thomason, L. Thacker, and R.H. Weaver. 1978. 

 Detection of Legionnaires' disease bactena by direct immunofluorescent staining. J. Clin. Microbiol. 8:329- 

 338. 



EnvironmenUil isolate 



102 



