Appendix 



Page 

 I. Chromogenic Cephalosporin Test for 



i3-LactaiTiase Production 154 



II. Cultivation of the Legionnaires' Disease 



Bacterium in Yolk Sac of Embryonated Hens' Eggs 156 



III. Detection of the Legionnaires' Disease Bacterium 



in Clinical Specimens (Flow Chart) 157 



IV. Blood-Freeze Storage of Bacteria 158 



V. Immunotluorescence Buffers and Mounting Fluid 159 



VI. Normal Chicken Yolk Sac (NYS) 160 



VII. McFarland Nephelometer Density Standards 160 



153 



