Physiology: Characteristics in Semisynthetic and Chemically Defined Liquid Media 



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Figure 7. Morphological aspects (1350 X) of the Philadelphia 1 strain of the Legionnaires' disease bacterium 

 grown for: 



A. 3 days at 37°C in 0.8% casein hydrolysate semisynthetic broth with shaking in air. 



B. 4 days at 37°C in 0.8% casein hydrolysate semisynthetic broth with shaking in air + 5% COj . 



C. 4 days at 37°C in 0.4% casein hydrolysate semisynthetic broth with shaking in air + 5% CO2 . 



D. 7 days at 25°C on the semisynthetic agar slants (0.8% casein hydrolysate); incubated in air. 



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