Laboratory Services 



2. Isolation from Clinical Specimens 



Portions of lung specimens submitted for isolation of possible LDB should be 

 carefully selected. To the extent possible, pathologists should select areas of dense 

 consolidation or areas with caseating necrosis, because such lung tissue is most likely to 

 contain LDB. Specimens should be packaged and mailed according to the Interstate 

 Quarantine Regulation (42 CFR Part 72) for shipping etiologic agents and should 

 be surrounded by enough cold-pack artificial ice to keep the material cold until it 

 arrives at CDC. Shipping specimens frozen or in dry ice is not necessaiy. Tissue fixed in 

 Fomialin or other fixatives is obviously unsuitable for isolation attempts but will be 

 examined with direct immunofluorescence. 



Sputa or other respiratoiy tract fluid specimens, except pleural fluids, cannot be 

 accepted for isolation attempts unless authorized in advance. 



Address requests and specimens to: 



Center for Disease Control 

 Attention: Dr. Roger McKinney 



Immunofluorescence Section 



DASH -Unit 11 



Analytical Bacteriology Branch 



Bureau of Laboratories 

 Atlanta, Georgia 30333 

 Telephone (404)329-3563 



3. Direct Immunonuorescence (IF) Test 



In addition to the two reference diagnostic services described above, the Bacteriol- 

 ogy Division provides reference diagnosis and consultation on the use of specific im- 

 munofluorescent conjugates for the direct staining of LDB in fresh or Fomialin-fixed 

 tissues and other clinical specimens. Neutral Formalin is the best fixative to use if the 

 tissue is to be examined with direct IF. Such tissues do not require refrigeration and 

 should not be frozen because they are then unsuitable for pathology studies. 



Address requests and specimens to: 



Center for Disease Control 

 Attention: Dr. Roger McKinney 



Immunofluorescence Section 

 ^ DASH-Unit 1 1 



Analytical Bacteriology Branch 



Bureau of Laboratories 

 Atlanta, Georgia 30333 

 Telephone (404) 3 29-3563 



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