FiTCHETT. — Physiological Action of Tutin. 



357 



P.M. 



3.32. 



3.42. 



3.45. 



3.46. 

 3.47. 



3.50. 

 3 52. 

 3.54. 



4.4. 

 4.5. 



4.6. 



4.6^. 



4.7." 



More convulsions, with lashing of tail ; movement of tail sometimes occurs quite 



apart from any movement in fore part of body, but occurs also with marked 



regularity at the beginning of each fit of fore part. 

 Several movements of tail, with no convulsion of fore part ; tail sometimes moved 



from root and sometimes only the tip ; movement is mainly from side to side, 



but now and then the whole tail i s lifted upwards. Reflexes present in the hind 



part ; movements of both limbs on tapping one. 

 Movement of tail becoming more marked, while fore part more quiescent. 

 Defgpcation occurred. 

 Hind limbs spasmodically dra\vn up to abdomen several times ; then a slight 



lit of fore part occurred, and movements of tail, with tetanic movement of 



hind limbs. 

 Tonic and clonic spasm of hind limbs ; claws extruded. 

 Respirations slow and gasping. 

 Heart-beat vigorous ; as heard by stethoscope, 72 per minute. Twitches of fore 



\rA\\s and tonic spasm of hind limbs, with erection of hairs of tail. 

 Clonic movements of left hind limb. 

 Distinct tonic and clonic spasm of both hind limbs ; limbs fully extended, with 



claws extruded ; respiration infrequent and difficult, owing to obstruction of 



air-i)assages by saliva and mucus ; heart intermittent, mis.ses one or two beats 



towards the end of inspiration. 

 More movements of hind limbs, fore part motionless; respirations about four 



])er minute. 

 Another spasm of hind c|uarters. 

 Respirations ceased : heart audible by stethoscope, beating regularly for two 



minutes and a half after last respiratory gasp. 



Exp. 154. 



A frog {Hyla aurea), jjithed in the ordinary way three hours previously, was sus- 

 pended by the head. At intervals of a few minutes its feet (as far as the ankle) were 

 dipped into dilute sulyihuric acid, of a strength of 1 in 1,000. After each dipping the feet 

 were carefully washed in fresh water. The time was taken by a seconds-clock, and the 

 result was as follows : — 



Fro2 A 



Average . . . . . . 6" 6" 



6-3 minims of a 0-1-per-cent. solution tutin (11 mlgm. per kilo) was then injected 

 under the skin of the back, and after an interval of 30 minutes the feet were again dipped 

 in the acid : — 



Right Foot. Left Foot. 



Frog A .. .. .. ..5" 4" 



4" 4" 



..6" 6" 



..8" 8" 



Average . . . . 5 '8" 5-5'' 



Here a further dose of 10 minims was injected, and after an interval of 15 minutes 

 the feet were dipped as before : — 



Eight Foot. Left Foot. 



Frog A . . . . . . . . 4" 4" 



4" 4" 



4" 4" 



4" 4" 



Average . . . . . . 4" 4" 



Each time the feet were withdrawal together, and, it was thought, more actively tlian 

 before the tutin was injected. 



