FiTCHETT. — Physiological Action of Tutin . 345 



Exps. 49, 50, 51. 



Three frogs. Weights : (1), 23 grams (Exp. 49) ; (2) 39 grams (Exp. 50) ; (3), 30 grams 

 Feb. 18. (^^P- ''^- 



P.M. 



6.20. To frog (1) was given 7 minims of a 0-05-per-cent. solution tutin (9 mlgm. per kilo). 

 ().24. To frog (2) was given 13'2 minims of a 0-05-per-cent. solution tutin (10 mlgm. per 



kilo). 

 6.31. To frog (3) was given 11-2 minims of a O'OS-per-cent. solution tutin (11 mlgm. per 

 kilo). 

 Ill each case the solution was injected under the skiu of the back, and the frogs 

 were placed in favourable suiroundings. 

 Feb. 19. 

 P.M. 



4.0. Frog (1) affected; Ij'iug prone on belly, with legs extended ; great muscular 

 weakness. Frog (2) presented the same appearance. Frog (3) was found dead 

 at 9 a.m. 

 Feb. 20. Frogs (1) and (2) apparently normal. 

 ■ ,, 21. Both frogs quite well. 



Exp. 52. 

 Feb. 20. Frog. Weight, 40 grams. 



P.M. 



5.0. Gave 14 minims of a 0-05-per-cent. solution tutin (10-25 mlgm. per kilo). The 

 solution was injected undei the skin of the back, and the frog placed in a cool 

 cellar, with moist grass and earth. 

 Feb. 21. 



P.M. 



2.0. Sprawling on belly ; hind legs extended ; abdomen distended ; twitching of toes ; 

 fibriliary twitching of muscles of thighs. On being handled, was seized with a 

 convulsion, affecting chiefly the hind legs ; the mouth was opened wide and 

 kept open, the animal croaking loudly. 



4.25. Dead. 



Exp. 53. 



P.M. Pigeon. Weight, 335 grams. 



5.30. Gave 5-6 minims of a 0-o-i3er-cent. solution tutin (5 mlgm. per kilo). The injec- 

 tion was made under the right wing. 



5.45. Very sleepy-looking ; narcotic effect is most marked ; tremulous about head ; 

 retching. 



5.50. General convulsions. 



6.5. General convulsive movements have continued since last note ; are now becoming 

 feebler; bird on its back. 



6.12. All movement ceased ; dead. 



Result. — Death in 42 minutes from a hypodermic dose of 5 mlgm. pei kilo. 



Exp. 54. 

 P.M. Frog. Weight, 20 grams. 



4.55. Gave 6-8 minims of a 0-1-per-cent. solution tutin (20 mlgm. per kilo), under the 



skin of back. 

 5.0. Breathing exaggerated ; keeps opening mouth wide. 



5.15. No further .symptoms ; gave other 7 minims. 



5.16. Rapid breathing ; mouth-opening movements. 



5.26. Gave other 7 minims. 



6.0. Lying prone on belly ; shows occasional slight twitchings, chiefly of hind legs ; 



if touched, is seized with conv^ulsions, and pupils dilate ; respirations irregular 



and exaggerated ; makes a sucking noise occasionally. 

 6.9. Turned on back ; cannot recover, and struggles violently and continuously in its 



efforts to do so. 

 6.11. Most severe convulsions; mouth spasmodically opened to fullest extent; cries 



loudly ; spasms occur every 4 or 5 seconds. (This change has occurred since, 



and apparently as a result of, violent voluntary efforts to recover position when 



placed on back.) Heart can be seen beating through abdominal wall ; rate, 



44 per minute. 



