344 Transactions. 



Exp. 44. 



Jan. 22. Frog. Weight, 40 grams. 



P.M. 



3.0. Gave 9-5 minims of a 005-per-cent. solution tutin (7 mlgm. per kilo), injected 

 under the skin of the back. The frog was placed under a bell-jar, with wet 

 gi'ass, in a cool cellar. 

 4.45. Api^arently normal. 

 6.30. No obvious effect. 

 10.30. No obvious effect. 

 Jan. 23. 



A.M. 



9.0. No obvious effect. 



P.M. 



4.30. No obvious effect. 

 Jan. 25. 



A.M. 



9.30. Apparently quite normal. 



Exp. 45. 

 Jan. 28. Frog. Weight, 18 grams. 



P.M. 



3.35. Gave 5'5 minims of a 0"05-per-cent. solution tutin (9 mlgm. per kilo), injected 

 under the skin of the back. It was placed in a cellar, with moist grass and 

 earth. 



6.10. Not normal ; squats fiat on belly ; muscular weakness of hind legs ; respiration 

 exaggerated. 



7.30. Hind limbs show spastic spasms ; unable to progress ; lies sprawling on belly. 

 Jan. 29. 



A.M. 



10.45. Seems much improved ; sits in normal position ; hops away unsteadily when 



touched. 

 Jan. 30. Seems weak, but in other respects is quite normal. 



Exp. 46. 

 Jan. 29. Frog. Weight, 38 gi-ams. 



P.M. 



5.33. Gave 13 minims of a 0*05-per-cent. solution tutin (10 mlgm. per kilo), injected 

 under the skin of the back. It was placed in a cool cellar, with moist grass. 



6.10. No change. 



7.9. Apparently vinaffected. 



9.40. Frog foimd in severe spasm, chiefly affecting the hind limbs, which are fully 

 extended ; is very excitable ; gave cries when touched, and climbed to the top 

 of the bell-jar ; no muscular weakness manifest. 

 Jan. 30. 



A.M. 



9.0. Very lively and excitable ; no distinct convulsions when touched ; is still noisy. 



P.M. 



2.30. Just dying ; mouth open ; could not obtain reflexes. 

 3.15. Dead. 



Exp. 47. 



Frog. Weight, 36 grams. 

 Jan. 31. Gave 11 minims of a 0'05-per-cent. solution tutin (9 mlgm. per kilo), injected 



imder the skin of the back. It was placed in a cool cellar, with moist grass. 

 Feb. 3. Quite normal. 



Exp. 48. 



Feb. 3. Frog. Weight, 31 grams. 



P.M. 



6.15. Gave 10*5 minims of a O'Oo-per-cent. solution tutin (10 mlgm. per kilo), injected 

 imder the skin of the back. As before, it was placed in favourable surround- 

 ings. 

 Feb. 4. 



P.M. 



3.45. Seems weak, but shows no marked symptoms. 

 Feb. 5. Apparently quite normal. 



