Fttchett. — Phijsiological Action of Tutm. 353 



J a 1. 26. The seeds were resown in the tin boxes. 



,, 30. All have grown more or less. The seeds soaked in normal saline have made- 

 most progress, and ai-e closely followed by those soaked in 0-1 per cent, and 

 0-2 per cent. Those soaked in 0-4 per cent, and 0-5 per cent, are growing, but 

 only two or three seeds in each, and the growth is not so vigorous as in the 

 others. Since the 26th the felt in the boxes has been kept moist by the 

 addition of water. 

 Feb. 3. All the seeds soaked in normal saline have grown, and are growing vigorously. 

 In the boxes labelled ''0-1 per cent." and "0-2 per cent." three or four seeds 

 are growing vigorously, and have made nearly as much progress as in the box 

 labelled " normal saline," but the others remain stunted, and one or two have 

 made no progress since being resoaked in the tutin solution. In the boxes 

 labelled "0-4 )ier cent." and "0'5 per cent." one or two seeds have made 

 good progress, but most of the others are stunted, or have not grown at all. 



Exp. 126. 



A few bars were taken from the gill of a cockle, and mounted in sea-water, and 

 observed xnider the low power. 



P.M. 



4.0. Normal saline perfused under cover-glass. 



4.35. No appreciable difference. 



4.38. 0-1-per-cent. tutin solution perfused under cover-glass. 



4.45. No change. 



4.46. 0-2-per-cent. solution tutin perfused under cover-glass. 



4.50. Seems to be a slight retardation, but doubtful. 



4.51. 0-3-per-cent. tutin solution ])erfused under cover-glass. 



4.52. Obvious slowing ; many cilia stationary, others moving feebly. 

 4.59. Ciliary movement practically ceased. 



Exp. 127. 



5.10. Fresh specimen taken in 0-3 per cent. 



5.11. Movements slower. 

 5.13. Very feeble movement. 

 5.15. ^lovements ceased. 



Normal saline and afterwards sea-water were then perfused under cover-glass, biit 

 recovery did not take place. 



Exp. 128. 



5.30. Fresh specimen taken, and a 0-2-per-cent. solution tutin perfused under cover- 

 glass. 

 5.37. Movements have practically ceased, except for a few cilia here and there. 

 5.45. All movement apparently ceased. 



A solution of j-^-^^ KOH in sea-water perfused under cover-glass, and ciliary 

 movement immediately resuscitated. 



p.m. Exp. 129. 



5.54. Fresh specimen taken, and a 0-2-per-cent. solution tutin applied. 



6.5. Cilia still moving ; movement confined to the apices of the cilia. A di'op of 



0-3-])er-ccnt. solution tutin applied. 

 6.15. Still moving, but less actively. A drop of 0-5-per-cent. solution tutin applied. 

 7.30. Still moving. 



As this piece of gill was rather large, a fresh specimen was taken from a mxissel. 



P.M. Exp. 130. 



7.45. 0-2-per-cent. solution tutin ajjplied. 



7.52. A distinct slowing has occuiTed, biit the cilia are still able to cause a movement 

 of particles. This only occurs in sheltered places. The cilia are at a standstill 

 on the edges. 

 7.57. A drop or two of sea-water applied, and many cilia restored to action. 

 In every case a control mounted in sea-water was used for comparison. 



Exp. 131. 



Two or three gill-bars from a small rock-oyster were taken and mounted in sea- 

 water raider a cover-glass. Two preparations were made, and one used as a 

 control. (Low power.) 



12— Trans. 



