FiTCHETT. — Physiological Action of Tutin. 351 



A.M. 



10.40. Blowfly very lethargic ; walks heavily ; can be pushed along without offering 

 to fly ; every now and then one or other wing extejided to full extent, and 

 held there a moment ; this is not a voluntary movement ; does not seem to 

 have proper control of legs when walking — one or more legs move irregularly, 

 and not in a suitable direction. 



11.0. Blowfly allowed to escape from cage ; remained in one position on table till 



touched, and then flew heavily on to window, crawled into a corner, and was 



siibsecpiently lost sight of. Two other blowflies introduced into cage ; one 



immediately fed from the watch-glass, and in 15 minutes showed symptoms; 



lethargic, and disinclined to move. 

 P.M. 



12.0. Second blowfly has been observed to drink but sparingly. First blowfly show- 

 ing moie marked symptoms ; tonic spasms of wings and uncontrolled move- 

 ment of legs ; allowed to escape, but on attempting to fly seized with a 

 general convulsion, buzzing round and round on back ; was attacked with 

 seizures of this kind imtil exhausted. 

 1.0. Lying on side, dead. Quite a considerable drop of clear fluid has exuded from 

 proboscis. 

 Mar. 10. All the house-flies are dead, and all show marked distention of abdomen. 

 Blowfly apparently luiafl'ected, so allowed to escape ; flew about on window 

 for few minutes, and was then seized with convulsions and died, as the others 

 had done. 



Exp. 117. 



Jan. 30. At 4.30 p.m. some mince was allowed to become infected by blowflies. A 

 small portion of the meat containing the larvae was placed in each of four 

 watch-glasses, and covered with a 001-per-cent., a 0025-per-cent., and a 

 005-per-cent. solution tutin, and with normal saline respectively. In each 

 case the larvae began to wander and coil up in the fluid. After one hour 

 these fluids were poured oft', and each watch-glass covered with another, to 

 prevent evaporation. 

 ,, 31. At 4.45 p.m. the watch-glasses were examined. In the one treated with 

 normal saline only the larvae ai'e numerous, have grown, and are very active, 

 crawlmg all over the surfaces of both watch-glasses. In the 001-per-cent. 

 and 0.025-per-cent. solution only one or two larvae are to be seen in move- 

 ment ; they are very small, and have not wandered from the mince. In the 

 005-per-cent. solution no movement is to be seen. 



Exp. 118. 

 A drop of hay-infusion, containing paramcecia, amcebae, monad.?, and bacteria, 

 was placed on each of four slides, labelled A, B, C, D. 



P.M. 



5.49. One drop normal saline added to B. 



5.50. One drop of a 01-per-cent. solution tutin in saline added to C. 



5.51. One drop of a 01-per-cent. solution quinine hydrobromate in normal saline 



added to D. 



6.5. B : No change. C : No change. I) : No paramcecia to be seen, but monads 

 and bacteria still moving. 



6.30. B : No change. C : Paramcecia may still be seen moving, but their move- 

 ments are irregular ; the monads are collecting at the surface and edge of the 

 drop; this may also be seen in A and B. Another drop of a 01-per-cent. 

 solution added to C. D : No movement. 



6.50. A and B : No change. C : Still one or two paramcecia moving ; are not so 

 easy to find ; many monads still active, others stationary ; amoebae still 

 moving ; field does not look .so lively as it did. 



Exp. 119. 



7.30. A drop of hay-infusion placed on a slide, and covei-ed with a cover-glass, and 

 a drop of a 01-per-cent. solution tutin normal saline placed at edge of cover. 



7.35. 8ome paramcecia have swum out into dro]i of tutin solution ; at once display 

 irregular movements ; remain in the drop of tutin solution. 



7.40. Movement of paramcecia in drop of tutin solution, which at first were excited, 

 now becoming slower and very irregular ; they roll over and over like rotifers. 



7.45. Paramcecia at a standstill in tutin solution : they appear to disintegrate in- 

 ternally, and are no longer recognisable ; paramcecia under the cover-glass 

 quite normal. 

 Experiment repeated, using a 0-5-{3er-cent. solution tutin in distilled water, with 

 similar results. 



