FiTCHETT. — Physiological Action of Tiitin. 343 



Exps. 36, 37, 38. 



Thi-ee frogs (Hyla aurea). Weights: (1) 27-5 grams (Exp. 36); (2) 28-4 grams 

 Jan. 15. (Exp. 37) ; (3) 33-7 grams (Exp. 38). 



P.M. 



4.25. Gave to frog (1), 4-1 minims of 0'01-per-cent. solution tutiu (1 mlgm. per kilo); 

 to frog (2), 3'4 minims 0'025-per-cent. solution tutin (2 mlgm. per kilo) ; to 

 frog (3), 6-8 minims 0'025-per-cent. solvition tutin (3 mlgm. per kilo). In each 

 case the solution was injected into the abdominal cavity, and the frogs placed 

 vmder bell-jars on the laboratory-table. 

 7.30. Frog (3) seems affected ; crouches in a sitting-position, and is blown out with air. 

 No apparent abnormality in frogs (I) and (2). 



10.0. No sign of convulsion in any when stimulated. Frog (3) seems more excitable 

 than the others. 



Jan. 16. 



A.M. 



8.55. All three appear to be affected ; are sluggish, and lying prone on their bellies, with 



legs extended. Frog (2) is almost dead. 

 10.0. Frog (2) dead (2 mlgm. per kilo) ; died between 9 and 10 a.m. 



Jan. 18. Frog (1) found dead in the morning (1 mlgm. per kilo) ; weight, 18 grams, a loss 

 of 9 grams. Frog (3) (3 mlgm. per kilo) is still alive, and seems quite normal. 

 No sign of convulsion noticed in these frogs. The two that died seemed extremely 

 thin and emaciated. Frog (1) looked very much thinner on the 17th than on the two 

 days before. The weather was cold on the 16th. On the 17th the temperature was 

 12° C. in the morning, but this does not explain why frog (3), with 3 mlgm. per kilo, 

 seems unaffected. Loss of weight might be due to drying ; but the air has not been 

 drj'. The weather is cold and wet. 



Exps. 39, 40, 41. 



Thi-ee frogs. Weights: (1) 17-3 grams (Exp. 39); (2) 25-1 grams (Exp. 40); 

 Jan. 16. (3) 32 grams (Exp. 41). 



P.M. 



4.40. Gave to frog (1), 2-6 minims of a 0'01-per-cent. solution tutin (1 mlgm. per kilo) ; 

 to frog (2), 7"5 minims of a 0"01-per-cent. solution tutin (2 mlgm. per kilo); 

 to frog (3), 5-7 minims of a 0-025-per-cent. solution tutin (3 mlgm. per kilo). In 

 each case the solution was injected into the abdommal cavity, and the frogs 

 placed under bell-jar on the laboratory- table. 

 10.0. No change observed. 



Jan. 17. No change observed. 

 ,, 18. Frog (2) was foimd dead in the morning ; weight, 18 grams. Weather cold. 

 Frogs (1) and (3) were ajiparently normal. 

 In the two experiments above, the number of minims m 1 c.c. was taken as 15 ; in 

 the subsequent experiments as 17. The doses per kilo body- weight above are therefore 

 smaller than as stated. 



Exps. 42, 43. 



Two frogs. Weights : (1) 31 grams (Exp. 42) ; (2) 41 grams (Exp. 43). 

 Jan. 21. 



P.M. 



5.0. Gave to frog (1),- 4 minims of a 0"05-per-cent. solution tutin (4 mlgm. per kilo). 

 5.15. Gave to fi'Og (2), 7 minims of a 0-05-per-cent. solution tutin (5 mlgm. per kilo). 



In each case the solution was injected under the skin of the back, and the frogs were 

 placed imder bell-jars, with moist grass and earth, in a cool dark cellar. 

 7.30. Frog (1) gave a long cry when touched; seems very excitable. Frog (2) 



apparently normal. 

 10.30. Could detect nothing abnormal in either. 

 Jan. 22. 



A.M. 



9.30. Both apparently well. 



P.M. 



2.30. Both quite normal. 

 Jan. 25. Quite normal. 



WiAl 



