346 Transactions. 



P.M. 



6.15. Respirations ceased. 



6.20. Heart-movement not so visible ; rate, 32 per minute. ]\Iuscles still show occa- 

 sional twitches and spasms. 

 6.30. Seems quite dead ; no reflexes. 



This frog received about 60 mlgm. per kilo. 



Exp. 55. 



p jj Frog (female). Weight of frog, 38 grams ; ovaries, 57 grams. 



4.12. Gave 1 minim saturated solution tutin. injected under skin of back. 



4.30. Remains crouching ; if turned on back, recovers position slowly. 



4.35. Turned on back ; recovers position with difhculty ; convulsive movements fore 

 and hind limbs ; lies prone on belly ; if legs extended, does not retract them ; 

 respiration feeble and irregular ; convulsive movements of limbs if turned on 

 back. 



4.40. Attempted to crawl, but unable to advance ; pricking skin of limb, no response. 



4.45. Respiration imperccjjtible ; if turned on back, recovers position with dilhcidty, 

 then tonic spasm in extension and a few vigorous respirations. 



4.55. \\Tien turned on back, quickly recovered, and made three or four vigorous hops ; 

 crying loudly, with jaws spasmodically 0]>ened, then lay on belly, and at inter- 

 vals of 10 to 15 seconds opened jaws widely and extended limbs in tonic spasm. 



5.15. \Mien turned on back, laj' so, occasional tonic spasm of fore limbs, irregular 

 feeble movement of hind limbs ; respiration at rare and irregular intervals — 

 two or three respirations, and then a more or less prolonged pause ; helpless, 

 lying on belly, reflexes still present ; makes no attempt to swim when placed in 

 water ; unable to rectify position in water ; lying on back at bottom of basin. 



6.7. Under bell-jar, makes occasional kicking-out movements ; still on belly, and 

 swollen-looking. 



7.40. Very similar ; makes less movement than before ; lies in practically any position 

 if gently handled, but reflex action still marked ; pupils dilated. 

 10.15. No spontaneous movement, and no reflexes (some movements, got on turning 

 over or letting fall, seem due to direct stimulation of muscles) ; pupils dilated ; 

 no respiration seen. 

 Next day, 9 a.m. Dead ; stifl: in position it was in for greater time after injection. 



Exp. 56. 

 P.M. Frog (small). 



3.0. Gave 1 minim semi-saturated solution tutin, injected under skin. 

 3.3. Attempting to jump, but movements already enfeebled. 

 3.15. Respiratory movement exaggerated ; abdomen distended. 

 3.20. Slowly extended hind legs and advanced fore legs, and lay on belly ; respiration 



quickened and exaggerated ; occasionally raises fore part of body in spasm to 



full extent of fore arms, and respiration ceases. 

 3.25. Twitching of toes. 

 3.35. Attempting to crawl flat on belly ; unable to make progress ; conjunctival reflex 



active ; skin reflex sluggish ; pupils dilated. 

 3.40. Spasm of all four limbs ; restless; raises body stiflSy, with hind limbs extended. 

 3.45. Continuous struggling-movements ; twice turned on back, and slowly and with 



difficulty recovered position. 

 3.50. Placed on back, unable to recover ; twitching of muscles and successive tonic 



spasms ; respiration rare and convulsive ; has opened mouth spasmodically 



once. 

 4.10. Reflex action abolished, except conjunctival, which is sluggish. 

 4.15. Repeated spasmodic opening of mouth. 



4.. 30. Can still see ; lies on back ; occasional movements of fore limbs. 

 5.5. On letting fall, slight reflex in fore limbs ; conjvmctival reflex still present. 



Exp. 57. 



P.M. Frog (small). 



4.30. One drop of a semi-saturated solution tutin in conjunctival sac. 



4.45. Movements of fore limbs to eyes ; conjunctival irritation ; restlessness ; ex- 

 aggerated respiration. 



5.0. Opisthotonic movements ; weakness of liind limbs ; crawls slowly ; hops with 

 difficulty. 



