FiTCHETT. — Physiological Action of Tutin. 347 



P.M. 



7.5. When light turned on. was foiind to be in a .state closely resembling clonic stage 

 of epileptic fit ; this soon subsided, but could be initiated to a lesser degree by 

 pulling animal about by hind leg. Pupils wide ; respiratory movement excited 

 and irregular. 

 10.30. Dead. 



Exp. 58. 



P.M. Frog (small). Weight, 12*5 grams. 



12.35. Gave 1 minim of a 0-05-per-cent. tutin solution in 0'76 per cent, saline, injected 



mider skin. Motionless, on bellj^. 

 12.42. First movement, lifted head. 

 12.45. Sprang forward ; increased respiratory rate. 

 12.50. Attempted to spring, but moved sluggishly, and did not advance ; emptying of 



cloaca, urine, and faeces. 

 12.55. Raised on all four limbs, and sank slowly back on belly. 

 1.0. Raised himself on all-fours, and remained sitting up with fore limbs extended ; 

 irregular crawling-raovements, chiefly in fore limbs ; feeble attempts to spring ; 

 hind limbs seem weak ; pupils dilated. 

 1.10. Restless ; attempting to crawl up side of bell- jar ; movements incoordinate and 



feeble. 

 1.43. Sitting motionless; pupils more dilated than at beginning of experiment; can 

 leap fairly well when toe pinched, but seems feebler, and does not resent inter- 

 ference so much as in a normal frog. 

 2.3. Leaning up against side of bell-jar ; left hand partially closed, as if grasping a 



twig ; right extended against glass. 

 2.43. Lying quiet ; recovers position fairly quickly when turned on back ; also shows 



occasional voluntary movements. 

 3.13. Same; pupils wider than previously. 

 4.13. As before, but respirations (taken from movement of floor of mouth) 40 for one 



half-minute, 30 for another half-minute, and rather irregular. 

 4.50. Lying prone on belly, with legs extended from sides ; if turned on back, recovers 

 with difficulty ; able to advance across table by short halting leaps ; hind legs 

 drawn up slowly to jumping-position after each effort ; no convulsive move- 

 ments noted so far ; seems to be a general muscular enfeeblement. most marked 

 in hind legs. 

 5.0. Makes continued efforts to jump, but fails to advance, as cannot flex legs suffi- 

 ciently beneath body. 

 CO. Lying prone on belly ; turns over when placed on back, though with difficulty. 

 7.35. Ljnng sprawling on belly ; limbs extended ; moves feebly when disturbed ; pupils 

 dilated (? due to darkness) ; while being observed it had something like a weak 

 convulsive fit. It lay on belly and kicked out repeatedly but feebly with hind 

 legs, and moved fore limbs as if attempting to swim. 

 9.35. Sprawling on belly as before, and motionless till distiu'bed. Weakness more 

 marked ; cannot turn over when placed on back ; makes several efforts, accom- 

 panied by deep breathing, and then lies still. 

 Next day, 9 a.m. Found dead, in same position as left in last night ; pupils firmly 

 contracted ; lower limbs extended ; fore limbs flexed, and digits closed, as if 

 grasping twig ; no swelling of abdomen. 

 P.M. — Gall-bladder distended ; intestines distended ; cloaca full ; intestine, some 

 dark grumous licpiid; stomach empty, except for mucus; kidneys apparently normal; 

 no abnormality at seat of injection ; central nervous system nothing abnormal, except 

 seems too pale ; no ecchymosis ; blood seems fluid, and animal as a whole seems more 

 bloodless than normal ; blood-film squeezed out of heart and tissues presents many 

 leucocytes, possibly dvie to mode of obtaining it. 



Exp. 59. 



P.M. 



5.45. Small trout, in 100 c.a water, with 10 drops saturated solution of tutin (about 



O'l per cent.). 

 5.49. Movement very excited ; breathing exaggerated. 

 6. 18. Breathing more laboured. 

 6.28. Swimming on side, near s\irface. 



6.30. Shuddering- movements ; spasmodic movements of gills, which then ceased. 

 Control, in same amount of water, showed no change. 



Exps. 60 to 76. 

 These experiments were very like the above (see Table III). 



