352 Transactions. 



Exp. 120. 



This experiment was essentially similar to Exp. 119. 



Exp. 121. 



Jan. 28. Six test-tubes wei-e taken and labelled — '' 0-5 per cent, tutin," " 0-4 j^er cent, 

 tutin," "0-3 per cent, tutin," "0-2 i^er cent, tutin," "01 per cent, tutin," 

 and " normal saline." The volume of fluid in each tube was the same. 



P.M. 



5.0. A small piece of fresh mmce was added to each tube, and the tubes left open 

 to the air. 

 Jan. 29. At 5-45 p.m. the tubes were corked, and placed in an incubator at 40° C. 

 „ 31. Tubes opened and examined. The tube containing normal saline was very- 

 offensive. None of the other tubes had the offensive smell of putrefaction ; 

 the smell was rather like that of stomach-contents. 

 Feb. 1. Contents of tubes examined under microscope ; all show moving bacteria, 

 but the drop taken from the tube containing normal saline was much more 

 crowded, and the bacteria in it showed a gi-eater variety of form and size. 



Exp. 122. 



Four fermentation-tubes were filled — (1) with glucose solution and tutin (0-25 

 per cent, of the latter) ; (2) glucose solution, with 0-1 per cent, tutin ; (3) 

 glucose solution alone ; (4) water. A few drops of a yeast emulsion were added 

 to each. 



P.M. 



5.45. Tubes placed in inciibator. 



7.10. Fermentation in (1) and (3). 



7.45. Fermentation in (2) as well, but only half as much gas developed as in (1). 



9.30. (]), (2). and (3) fully fermented. 



Exp. 123. 



4.40. Two fermentation-tubes set on — (1) with glucose alone ; (2) with 0'05 per cent. 



tutin. 

 5.10. No change. 

 6.10. Both equally fermented. 

 8.30. Both fully and equally fermented. 



Exp. 124. 



Four fermentation-tubes filled with the following : — A : Water (16 o.c), 25 per cent, 

 glucose (4 c.c.) ; B: Water (20 c.c.) ; C : Water (8 c.c), jilus 0-5 per cent, 

 tutin solution (8 c.c.) plus 25 per cent, glucose (4 c.c.) (equals 0-2 per cent, tutin 

 in mixed fluid); D: 0-5 per cent, tutin (16 c.c), plus glucose (4 c.c.) (equals 

 0-4 ])er cent, tutin in mixed fliiid). A few drops of freshly procured emulsion 

 of brewers' yeast added to each ; D received rather more than the others. 



P.M. 



3.30. Tubes placed in incubator. 



4.40. Small bubbles forming in A, C, and D. 



5.10. Same. 



6.0. Fermentation most distinct in D. 



7.10. Most fermentation in D ; slight in C ; little or none in A and B. 



7.30. Added another drop of yeast emulsion to A. 



8.0. Fermentation now as great in A as iii D. 



This shows that the amount of yeast added exerts a greater influence on the amount 

 of fermentation that occurs than does the presence of tutin. 



Exp. 125. 



Five test-tubes were taken, containing a 0-1-per-cent., a 0-2-per-cent., a 0-3-per- 

 cent., a 0-4-per-cent., and a 0'5-per-cent. solution tutin in normal saline ; and 

 one test-tube containing normal saline alone. Equal volumes of fluid in each 

 test-tube. 



Jan. 23. At 4.30 p.m. twelve miistard-seeds were placed to soak in each test-tube. 

 ,, 23. At 4.30 p.m. the seeds from each test-tube were sown on moist felt placed in 



separate tin boxes, correctly labelled. 

 ,, 24. All the seeds in each box have germinated. 

 ,, 25. At 10.10 a.m. the same progress has been made in each case. All the seeds 



were replaced in their respective sohitions. 



