354 Transactions. 



P.M. 



2.30. A drop or two of a 0-5-per-cent. solution in sea-water tutin perfused under cover- 

 glass. 



2.35. Appears as if there was a slight slowing of cilia in exposed situations, but doubtful. 



2.40. Cilia still moving ; more 0"5-per-cent. solution tutin i>erfused under cover-glass. 



3.0. Most cilia in active movement ; here and there a few are motionless, or moving 

 feebly. 



4.0. Still moving. 



Exp. 132. 



A fresh preparation was made from the gill of a cockle, and examined with the 

 low power without a cover-glass. A control was used. 

 P.M. 



3.10. A drop of a 0-5-per-cent. solution tutin a))plied. 

 3.12. If anything, movements seem exaggerated. 

 3.20. First drop tutin solution dried off. and a second drop added. 

 3. .50. No effect observable. 

 4.30. Still in active movement. 



Exp. 133. 



i Three preparations of ciliated epithelium from the gullet of a frog. No. 1 was 



mounted in normal saline without a cover-glass, and used as a control ; No. 2 

 was moiuited in a drop of a 0-1-per-cent. solution tutin, without a cover-glass: 

 No. 3 was mounted in a drop of a 0-5-per-cent. solution tutin, without a cover- 



! glass. Three microscopes were used, and the s])eciraens examined inider the 



low power, with the draw-tube out. 



only. 



Exp. 134. 



The gullet of a frog was exposed, and the rate of progress along it (by ciliary 

 action) of a small fragment of cork was observed. A centimeter scale was 

 arranged to lie parallel with the gullet, and the time the cork took to travel 



1 cm. noted as follows : — 



Gullet bcitlied with 

 Normal. a 03-per-cent. 



Solution Tutin. 

 52" 32" 



47" 38" 



61" 38" 



51" 29" 



51" 37" 



Average, .'iO-4" Average, 34-8" 



Exp. 135. 



Two frogs' nei've-muscle preparations made. Muscles placed in watch-glasses 

 with normal saline. Nerve laid on frog-plate, and kept moist with saline, and 

 stimulated with break shocks. A (in saline), minimal stimulus found to be with 

 coil at 17 cm. B (in saline), minimal stimulus at 21-5 cm. B was then placed 



solution tutin in normal saline 



