PRINCIPAL INVESTIGATOR(S ) : Takashi Hoshizaki, Ross Durham, and W. Ross Adey 



EXPERIMENT TITLE/NUMBER : Sleep/Wake Activity Patterns of a Macaca nemestrina 



Monkey During Nine Days of Weightlessness 



PROGRAM/MISSION : Biosatellite III 



CLASSIFICATION : Animal - Pig-tailed monkey ( Macaca nemestrina ) 



DISCIPLINED ) : Chronobiology 



OBJECTIVES : To study the possible effects of space environment on the 

 sleep/awake cycle of a pig-tailed monkey. 



PROTOCOL : Time-lapse photographic records of the flight and control animals 

 were taken in conjunction with other physiological data. Pictures were taken 

 every 20 minutes. The pictures were analysed for the cycle with the status of 

 the eyes, i.e. closed, open, not discernible. 



EQUIPMENT : 16mm movie cameras, 24 hour clocks. 



RESULTS : The subject was entrained to 24 hour cycle but with a shift of 2 

 hours sleep 1400-0200 CT , wake 0200 to 1400 CT. With the exception of the 

 last two days, the subject tended to remain on a consistent schedule regarding 

 onset of "night" sleep. He remained asleep for longer periods of time as the 

 flight progressed. Wakefulness apparently never increased in the "night" 

 mode, but increased during "day" mode on days 7, 8, and 9. 



CONCLUSIONS : "Bonny" changed rapidly back and forth from the sleep to wake 

 status. From the findings on a 24-hour rhythm in blood pressure, a 26-hour 

 rhythm in the heart rate, body and brain temperature and pC0~, and the 24-hr. 

 rhythm in the sleep/wake activity, it is concluded that internal desynchro- 

 nosis occurred in the flight subject. Desynchronosis may have contributed to 

 the physiological pathology that prompted the termination of the flight. Such 

 a desynchronosis was not observed in control subjects. 



PUBLICATIONS : 2, 4, 6, 7, 241, 285 



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