PRINCIPAL 

 INVESTIGATOR(S) 



Brian Palenik 



3200 Hubbs HaU 



Marine Biology 



Scripps Institution of Oceanography 



University of California - San Diego 



LaJolla, CA 92093-0202 



PROJECT TITLE 



CELL-SURFACE PROTEINS AS MARKERS FOR THE 

 PHYSIOLOGICAL STATE OF COASTAL 

 PHYTOPLANKTON 



AMOUNT OF FUNDING FY 1994: $143 K 



SUMMARY OF GOALS 



The goals of the sampling program are to 1) Begin to validate the use of polyclonal 

 antibodies to specific cell surface proteins as indicators of physiological state of the 

 phytoplankton. 2) Use the antibodies in more of a survey mode once they have been 

 characterized. It should be noted that essentially every identified cell surface protein/organism 

 combination will go through both phases. We expect to have at least two to three antibodies 

 ready to test in 1995. 



SPATIAL AND TEMPORAL SAMPLING SCALES 



The spatial and temporal scales will be relatively modest in phase 1 including only a few 

 depth profiles. We hope to work out conditions for using preserved cells which may be organism 

 specific. Phase 2 will involve a more systematic survey of the sampling area. Further information 

 on species distributions within the sampling site and isolation of specific strains will help in the 

 choice of sampling scales. 



METHODS AND PLATFORMS 



Possibly one liter samples from bottle casts will be required. The samples will be assayed 

 using fluorescently labeled antibodies and fluorescence microscopy on board ship and/or on 

 preserved samples in the laboratory. 



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