TABLE 4 (continued) 



Percentage 

 saturation 



140% 

 Cage B 

 Tank 1 



130% 

 Cage A 

 Tank 2 



130% 

 Cage B 

 Tank 2 



120% 

 Cage A 

 Tank 3 



120% 

 CageB 

 Tank 3 



100% 

 Control 

 Cage A 

 Tank 5 



100% 

 Control 

 Cage B 

 Tank 8 



Daphnia Time to Signs of gas bubble disease (CBD) 



No. death (hr) (B = Bubbles) 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 

 10 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 

 10 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 

 10 



1 

 2 

 3 

 4 

 5 

 6 

 7-10 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9-10 



1 

 2-10 



1-3 

 4-10 



53 No apparent GBD, fungus 



B in gut and brood pouch, fungus 



102 B under carapace 



119 Brood pouch full of air 



119 Gut full of air 



119 No apparent GBD, fungus 



150 No apparent GBD 



150 No apparent GBD, tissues fragmented 



150 No apparent GBD, tissues fragmented 



150 No apparent GBD, tissues fragmented 



77 No apparent GBD, fungus 



77 No apparent GBD, fungus 



102 Air in gut, 1 B under carapace, fungus 



150 No apparent GBD 



150 No apparent GBD, tissues fragmented 



169 No apparent GBD, tissues fragmented 



169 No apparent GBD, tissues fragmented 



217 No apparent GBD, tissues fragmented 



243 No apparent GBD, tissues fragmented 



lost Apparently disintegrated 2 



102 

 119 

 150 

 150 

 169 

 217 

 243 

 264 

 264* 



150 

 264' 



78 No apparent GBD, fungus 



78 No apparent GBD 



102 Gut full of air 



119 No apparent GBD 



119 No apparent GBD 



119 No apparent GBD 



150 Big B in brood pouch separating carapace halves, gut 1/2 full of air 



150 Large B separating carapace, gut full of air 



169 No apparent GBD 



198 No apparent GBD 



150 No B, fragmented, fungus 



150 No B, fragmented, fungus 



150 No B, fragmented, fungus 



150 1 small B under carapace 



243 B in gut and brood pouch 



264 No apparent GBD 



264* No apparent GBD 



No B, fungus 



No B 



No B, carapace torn 



No B, fragmented 



3 small B under carapace 



No apparent GBD 



2 small B under carapace 



No apparent GBD 



No apparent GBD 



Carapace torn 

 No apparent GBD 



150 3 lost or fragmented 



264* No apparent GBD 



'WFTS Test No. 34 



■Tungus begins growing on Daphnia soon after death 



#Alive after 11 days. 



Effects on Freshwater Invertebrates 57 



