100 

 80 



i M 



40 

 20 

 



100 

 80 



ft «> 



40 

 20 

 



100 

 80 



" 60 



in 



£ 40 

 20 







//y 



-140% 

 ,«1 1307. 



120% 



DAPHNIA 



r 



-END OF TEST 



160 



200 



240 



120 160 



TIME (HOURS! 



FIG. 5 Daphnia with air bubbles in the gut (upper and lower 

 animals); center animal without air bubbles is control. 



FIG. 6 Time to death for Daphnia magna exposed to 110%,, 

 115%, 120%, 130%, 140%, and 150% total dissolved gas saturation 

 (Test 1, 2 and 3). 



TABLE 4. Daphnia Mortality After 11 Days at 150%, 140%, 130%, 120%, and 100% Total Gas 

 Saturation of 12 C. and Observed Signs of Gas Bubble Disease' (Test 2). 



Percentage 

 saturation 



Daphnia 



No. 



Time to 

 death (hr) 



Signs of gas bubble disease (GBD) 

 (B = Bubbles) 



150% 

 Cage A 

 Tank 4 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 

 10 



72 No B, gut with food 



102 Gut full of air, B under carapace 



102 B in brood pouch and under carapace 



102 B in gut and brood pouch 



102 3 B under carapace 



102 B under carapace 



102 B uncer carapace 



102 B under carapace 



119 Small B under carapace 



150 No apparent GBD 



150% 

 CageB 



Tank 4 



1 

 2 

 3 



4 

 5 

 6 

 7 

 8 

 9 

 10 



52 No obvious B, some fungus 2 



72 B in gut and brood pouch 



102 B under carapace, fungus 



102 B in gut and brood pouch 



102 B in gut 



102 B under carapace 



119 Gut 3/4 full of air, B in brood pouch and under carapace 



119 4-5 small B under carapace 



150 5-6 small B under carapace 



150 No apparent GBD 



140% 

 Cage A 

 Tank 1 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 

 10 



53 Large B filling brood pouch, some fungus 



53 Large B in brood pouch 



102 Gut full of air, large B in brood pouch, B under carapace 



102 B in brood pouch and under carapace 



102 2 small B under carapace 



150 No apparent GBD 



150 Gut and brood pouch full of air 



150 1 B under carapace, some fungus 



150 No apparent GBD 



198 2 small B under carapace 



56 Nebeker, Stevens, Brett 



