2 6o The Ohio Naturalist. [Vol. II, No. 6, 



significant. 34.2 per cent, of the smut balls were either^;/ or 

 ivithin six incha, of the wound but within the next six inches 

 above and below the wound only 13.6 per cent were to be 

 found. 



Row No. 2. 



Each stalk (842 in all) was mutilated as in No. i and then 

 smut spores immediately brushed on or painted over the wound. 

 The smut used had been kept dry in a tight box since the fall 

 of 1900. The work was done after 5 p. m. and was followed by 

 a good dew but no rain soon. 



Results: 1 1.5 per cent, of these stalks were smutted against 

 but 8.5 per cent, in the check. The relative position of the smut 

 in this part of the experiment was, however, quite significant. 

 59.8 per cent, of the diseased stalks were infected within six 

 inches or upon the wound, while, in the next six inches above 

 and below the wound, only 17.5 per cent, of the bolls were to be 

 found. 



Rozc No. 3. 



Each of these 806 stalks was painted at some place with spores 

 as in No. 2 but none were mutilated. This was done in early 

 evening and although followed by no dew, a fine mist fell the 

 next morning. 



Results : 10.4 per cent, of the stalks were smutted against a 

 check of 8.6 per cent. Also the percentage of smutted ears to 

 total diseased stalk was again significant ; in the check-row 20.9 

 per cent, while in the infected row it was but 16.6 per cent. 



Roic No. /. 



This row was detasseled during the partly cloudy forenoon of 

 August 3. 



Results : 1 1.5 per cent, of the stalks were diseased against a 

 check of 9.5 per cent. The percentage of the total diseased 

 stalks having the ear as the affected part was 22.3 per cent, in 

 this row and 28.6 per cent, in the check- row. 



Stated very briefly the results are in accord with those obtained 

 at the Indiana and Illinois P^xperiment Stations in recent years. 

 At the stage of growth when the tassels are just appearing, detas- 

 seling, mutilation of the stalks lower down, and the application 

 of spores without wounding the stalk all cause an increase of 

 smut ; and mutilation and the application of spores to the wound 

 thus made results in a still larger per cent, of smut. 



