^7° The Ohio Naturalist. [Vol. II, No. 2, 



bur, rag-weed and wild-rice are given as their food-plants. In 

 some cases a single species of fly has been reared from a half 

 dozen or more different plants. Agromj'za setosa Loew, as deter- 

 mined by Coquillett, was reared in numbers from leaves of wild- 

 rice, Zizania aquatica, at Sandusk}- during August of each of the 

 years 1900 and 1901. Professor Osborn studied the species and 

 its work in 1900, while my observations were made a year later. 

 Although I include the notes taken by both of us, many points 

 are needed before a detailed account of the habits and life history 

 of the .'ipecies can be given. 



The eggs are conspicuous on account of their abundance and 

 white color, and are deposited chiefly on the upper surface of the 

 leaves of the food plant. 



The larvae upon hatching bore into the leaf and feed beneath 

 its upper covering. When full grown they measure about 6 mm. 

 in length, are white, or greenish on account of chlorophyl taken 

 in with their food, and are furnished with strongh- chitenous 

 mouth parts. The mines which they make in the leaves are 

 irregular in width and extend for varying lengths on one side or 

 the other of the mid-rib. These variations in extent are usually 

 explainable from the fact that a variable number of lavae occupy 

 the different mines. The work of the larvte is apparent from 

 the first on the upper side of the leaf, and may be seen beneath 

 after a few days because of the fact that the parts beneath the 

 mine sooner or later turn yellow. 



. The pupa is to be found either in the mine or clinging to the 

 surface of the leaf. It is brown in color, with two prominences 

 anteriorl}^ where the attachment with the leaf is effected, and is 

 contained within the last larval skin so that the legs and wing- 

 pads are at no time visible from the outside. 



BiBio ALBiPENNis Say — Larvae observed in colonies under 

 fallen logs, and boards which were lying on the ground. vSpeci- 

 mens taken April dth pupated May 5tli and the adults appeared 

 May 13th. The adults were unable to fly for several hours after 

 tliey emerged on account of their wings remaining soft. I ob- 

 served the first males flying out of doors on the 23d of May. 



Chrvsopila ornata Say — Larva about an inch and a half 

 in length, white in color, cylindrical, with an enlargement at the 

 posterior end bearing a number of fleshj^ elongations which are 

 about the length of their basal breadth. Found under rotten 

 wood May ist. Pupa brown, last segment armed with six 

 spinose teeth, the two on the ventral side arising from the same 

 base, the remaining abdominal segments furnished with a circlet 

 of spines near the posterior third. The adult emerged the i8th 

 of June. 



