162 TWO NEW INSTRUMENTS FOR BIOLOGISTS, 



As regards the mixtures used iu differentiating, I formerly 

 made them as they were required. I now keep a stock of fluids 

 on hand. My present stock I find to be as follows : — 



I. — The objects are to he mounted uncut in balsam. 



Alcohols of the following strengths :— 7%, 15%, 22%, 30%. 



Mixture three parts 30% alcohol and one part borax-carmine. 



Mixture one part 30% alcohol and one part borax -carmine. 



Mixture one part 30% alcohol and three parts borax-carmine. 



Borax carmine. 



Mixture three parts borax-carmine and one part 60% alcohol. 



Mixture one part borax-carmine and one part 60% alcohol. 



Mixture one part borax-carmine and three parts 60% alcohol. 



Alcohols of the following strengths :— 60%, 65%, 70%, 75%, 

 80%, 85%, 907o, 95°/„, 1007o, acidulated as may be necessary. 



Mixture of three parts absolute alcohol and one part clove-oil. 



Mixture of one part absolute alcohol and one part clove-oil. 



Mixture of one part absolute alcohol and three parts clove-oil. 



Clove-oil. 



Mixture three parts clove-oil and one part thin balsam. 



Mixture one part clove-oil and one part thin balsam. 



Mixture one part clove-oil and three parts thin balsam. 



Thin balsam. 



In all twenty-eight. 



II. — The objects are to be cut. 



Go to absolute alcohol as in I. 



Then, mixture three parts absolute alcohol and one part chloro- 

 form. 



Then, mixture one part absolute alcohol and one part chloroform. 



Then, mixture one part absolute alcohol and three parts chloro- 

 form. 



Chloroform. 



Then use Giesbrecht's method or some equivalent one for 

 imbedding in paraffin or all previous precautions against shrinkage 

 will have been useless. 



III. — Alcoholic carmine is to be used. 



Go to 60°/o alcohol as in I. 



