BY N. A. COBB. 163 



Then mixture three parts 60°/o alcohol and one part Mayer's 

 carmine.* 



Then mixture one part 60°/o alcohol and one part Mayer's 

 carmine. 



Then mixture one part 60° Jo alcohol and three parts Mayer's 

 carmine. 



Mayer's carmine. 



Mixture three parts Mayer's carmine and one part acidulated 

 absolute alcohol. 



Mixture one part Mayer's carmine and one part acidulated abso- 

 lute alcohol. 



Mixture one part Mayer's carmine and three parts acidulated 

 absolute alcohol. 



Absolute alcohol. 



Then the appropriate clove-oil and balsam mixtures. 



IV. — The objects are to be preserved in glycerine. 



Glycerine of the following strengths, lO^^, 207o, 30%, 40'7o, 

 50%, 60%, 707o, 80%, 90%, 100%. 



When turpentine and such other fluids as act on caoutchouc are 

 used, it becomes necessary to use stout caoutchouc tubing and to 

 tie it on flrmly. 



The Suction-capsule. 



The suction-capsule was devised to aid in solving those difficult 

 and important problems connected with the development in the 

 human alimentary canal of the eggs and larvae of the internal 

 parasites peculiar to man, and incidentally to serve in a similar 

 manner in investigating the internal parasites of the lower 

 animals. 



The usual procedure (feeding the eggs to a subject direct and 

 recovering them or the resulting larvte by killing the subject) is 

 not applicable to man, and is in any case open to some serious 

 objections, especially in certain cases. It is often impossible to 



* I make this carmine to contaia 80% alcohol and ensure the exact per- 

 centage of alcohol by making in a flask immediately connected with a 

 vertical Liebig's condenser. No alcohol is then lost by evaporation during 

 the boiling. 



