BV K. C. fJRKY. 313 



cipitate appeared ; water was now gradually added, and the 

 precipitate, which thus separated, was filtered and washed 

 with alcohol-ether mixture. 



25 cc. ox-blood gave 3-96 grams protein — 15-84 per cent. 



Data of Sulphur Determination. 



(1)0-2700 'rmm protein gave 0-0094 gram Ba.S.j, 8 = 047% 



(2)0-6460 gram protein gave 00200 gram BaSO, S = 0-43% 



Mean S^= 0457 



From this value for organically combined sulphur in 

 globin, it is possible, as shewn below, to calculate the dis- 

 tribution of the sulphur in globin sulphate, and also the 

 proportions in which the globin and the sulphuric acid stand 

 related. 



In globin sulphate. 



Organically combined sulphur 041% 



Sulphur as sulphuric acid 2-64% 



Total sulphur 3-05% 



Calculation of the composition of globin sulphate. 

 Assuming 100 parts globin sulphate to contain .r parts globin, 

 since, by experiment, globin contains 0-45 per cent. S. 



Then the amount of organically combined sulphui- in 100 parts 

 of globin sulphate is equal to 00045a;. 



Again 100 parts of the sulphate contain HOO-.r) of H.,SOj^. 

 Therefore, sulphui- present as H.-^SO^, is etjual to (]00-x)l^ 

 per cent. 



But ( 100-d^)i^ -t- 0-0045.r = total sulphur in globin sulphate; and. 

 by experiment, the total sulphur was found to be 3-05^' . 

 Hence (lOO-,/-)!;; -f 0-0045./-= 3-05 

 100-a.--f-0-0137.r = 3-05 



0-9863.r = 90-667 



.r = 91-92 

 Hence for globin sulphate : 



OJlobin 91-9-2 per cent. 



Sulphuric acid .. 8-08 ,, 



