BY JAMES M. PETRIE. Ill 



Table vii. 



N. 

 (i.) Total nitrogen in the protein-free solution 100 '0 



(ii.) Ammonia evolred in the cold by lime water 2'8 



,, distilled with magnesia 99 



(iii.) Pptd. by phosphotung.stic acid after distilln 182 



(v.) Sod. hypobrom. in phosphotung. -filtrate after distilln.... 19'3 



Undetermined ... 52 6 



(iii) Phoaphotungstic ppt. in orig. solution 20'2 



(iv.) ,, filtrate distilled with magnesia 1'13 



,, ,, hydrolyaed and ,, 4*23 



(4) The next series was carried out on a solution obtained 

 by method (b), protein precipitated by alcohol. 



The following diagram will serve to show the order of the 

 experiments. 



^^^nitrous acid. 

 Hydrolysis and distn. — ^ — hydrolysis and distu. — ^nitrous acid. 

 ~~~~^phosphotung. 



(i.) The solution was hydrolysed by boiling in 8% hydro- 

 chloric acid, for two hours, neutralised, and distilled with 

 magnesia, and titrated every hour. 



Table viii. 



After 1st hour 8-07% 



2nd „ 0-66 



3rd „ 0-46 



4th ,, 0-41 



4 hours. 9-6% of the N. 



(ii.) The same solution was then hydrolysed a second time 

 in 8% hydrochloric acid, for two hours, and the distillation 

 with the magnesia repeated. 



Table ix. 



Distilled i hour 5-2% 



i ,, longer 1-2 



b. M „ 0-8 



* „ „ 0-8 



8-0 % of the N. 



