307 



CONTRIBUTION TO OUR KNOWLEDGE OF THE 

 CHEMISTRY 0¥ BLOOD. 



No 1. Globin Sulphatk and Globin from Ox-Blood. 



By E. C. Grey, B So., Acting Dkmonstuatok and Assistant 

 Lkcturrr in Physiologv in thk Univkrsitv of Sydney. 



(From thf PJi>/siol(>(/ifaf Lahorcifor)/ of the University of Si/diiei/). 



General consideration is.— Oxyhsenuti'luliiii from ox-blood lias 

 received very littU' attention as regards its chemical composition, 

 and although, as is well known, this is due to the fact that the 

 substance is not readily cr^-stalli sable, nevertheless it is not a 

 little surprising in view of the fact that a solution of ox-blood is 

 universally accepted as a standard solution of hseinoglobin. The 

 fact that hseinoglobins behave differently in their properties, 

 especially as regards their power of crystallising, is a clear indi- 

 cation of a difference in chemical constitution, and hence in the 

 ease of ox-blood hfemoglobin we might well expect this difference 

 to be considerable. That such is the case is demonstrated by the 

 results put forward in this communication. The researches of 

 Kijster* and others have almost conclusively shown that these 

 differences are not due to any variation in the composition of the 

 prosthetic group, and moreover since this portion of the h;«mo- 

 globin molecule constitutes only five per cent, of the whole', it 

 cannot account for the marked differen<;es in haemoglobins fi-om 

 various sources. The difference must, therefore, certainly lie with 

 the albuminous moiety, and this is borne out by experiment. 



I have been able to show that the globin of ox-blood is more 

 basic than that from the haemoglobin of the horse, which has 

 served as the material of most previous investigations This 

 basicity is evidenced l)y the power of the histone to combine with 

 nearly nine per cent, of sulphuric acid. 



• H. S. Zeit. Physiol. Chem, Bd. 40, S. 391, 1904. 

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