BY K. r. r.REV. -il I 



lOOX'., and it is probable that, even when heated to lOO'C. for 

 many days, the p-eparation still contains water in sonic form of 

 combination >/ It is interesting,' to note, therefore, the nature of 

 the precipitate prepared liv very gradual dessication lasting about 

 two months, and at a temperature never exceeding 50X\ 

 The weights obtained were as follows : 



Globin sulphate from SOcc. detibriiiated ox-blood. 



(a) S-94 yranis. 



(b) 8-84 ,, 

 (e) 8-38 ,, 

 (<i) 8-34 „ 

 (e) S-48 „ 



It was found, unfortunately, that Nos. (a) and (h) contained 

 particles of glass, and they were, therefore, not included in 

 calculating the mean value. The last estimation. No. (e), was 

 carried out in two days b}' drying the globin in an open glass 

 vessel at llCC. 



The percentage of globin sulphate yielded by ox-blood is, 



therefore, 



Globin sulphate weighed Calculated to original 

 from lOOcc. ox-blood. globin. 



No.l lti-76 15-40 



No.2 16 68 1532 



No.3 16 94 15-58 



Mean 16 79 Mean 15-43 



The figures in the second column are found by deducting the 

 amount of combined sulphuric acid, viz. 1-36 grams determined 

 in the manner to be described later, from the figures of the first 

 column. 



There is a good indication, therefore, that the l^awrow method of 

 obtaining globin sulphate gives a product of constant composition. 



This is further borne out by the determination of the nitrogen 

 and sulphur in many specimens of the sulphate obtained under 

 various conditions, as i-egards the i-oiicentration of sulphuric acid 

 in the })recipitating Huid. 



ExPEKi.MENT 3. Thf sulphur in ijlobiii and globin sulphaf*^ 



from ox-blood. 



The total sulphur in the globin sul{)liate was determined by 

 moistening with a strong solution of KOH, evaporating to dry- 



