314 THE CHEMISTRY OF BLOOD, I. 



The ihfermination of the nitrogen in the sulphate of glohin 

 from ox-ldood, and ihf calculat/wii of the Hitrogen iit tha 

 original glohin. 



TIk- nitrogen was determintnt l)y K jeldalil : 



(1)0 2635 Kiain of sulphate of tjlohin required 



... -iT-ecc. .\710H„SO, N 14-62% 



{2)0 1902 giani ,, ,, ... 20 3cc. ,, ,, N 14-94% 



(3)0-3897 t;ram ,, ,, ... 41-2cc. ,, ,, N 14 80% 



(4)0-5863 gram ,, ,, ... 62-lcc. ,, ,, N 15 0% 



Mean of three determinations N 14'91% 



16-79 grams of globin siilphate are equivalent to 15-66 grams 

 of globin. 



Hence the percentage of nitrogen in globin N 15-98% 



Calculated to ash-free globin N 16-03% 



The percentage of nitrogen in globin from horse- 

 haemoglobin, according to Shulz* N 16-89% 



Determination of the ash. 

 02437 gram of globin sidphate gave 0-0008 gram ash, 0-32%. 

 The ash found by Shulz in globin from horse-blood was 

 0-58% 0-84 %. 



ffENEKAI. CONCLUSIONS. 



(1) Each hundred cc. of ox-blood yi(l<l 16-79 grams of globin 

 sulphate, which is equivalent to 15-43 grams of globin. 



(2) The globin from ox-blood is more basic than that from 



the haemoglobin of the horse. 



(3) The sulphate of globin precipitated from solutions con- 



taining varying concentrations of sulphuric acid is of 

 constant composition, containing 8-08% sulphuric acid. 



(4) The percentage of nitrogen found in the globin sulphate 



is 14-9, from which the calculated percentage of nitrogen 

 in the globin from the blood of the ox is 16-03%. 



(5) The globin precipitated by trichloracetic acid was found 

 to contain 0-45 per cent, sulphur. 



In conclusion. I beg to express my thanks to Professor Anderson 

 Stuait. ill whose laboratory this work has been done. 

 " *Hrsr ZeitTPhysiolT Chem. Bd. 24, S,449, 1898. 



