8 



FUNGOID DISEASES 



tures, and observing the changes from time to time 

 under the microscope. They may be made as 

 follows : — 



Upon a piece of ordinary glass 3|- in. x i in. 

 (Fig. 5, A) place a small piece of cardboard ^ (B) 



'SMi'lFl^i^'^^':^!^ 



Fig. 5. — Sketch showing how drop cultures are made, on which the ger- 

 mination of spores and growth of fungi may be observed direct under 

 the microscope. Also shown in section. Description in text. 



about one inch square, having a hole (C) in the 

 centre. Take a very thin piece of glass one inch 

 in diameter (cover glass) clean and sterile, and 

 place upon it, by means of a glass rod, a drop of 

 sterile water or other substance.^ 



Place the smallest possible number of spores on 



^ Boil the cardboard for one minute prior to using, and keep all 

 glass and instruments used as sterile as possible. 



^ Various substances are used, e.g. prune juice, dung extract, 

 sugar solutions, beer wort, etc. ; some spores germinating best in 

 one, some in another. All substances used must be germ free. 



