PASTEUPS, CORN'S, MIGUEL'S FLUIDS, ill 



modification for their cultivation ; and various slight alterations in its 

 composition render it of great value in the culture of several of the 

 other groups of micro-organisms. 



Pasteur's Fluid. 



59. Pasteur's fluid consists of — 



Distilled water, . . . iooo'o parts. 



Pure cane sugar, . . ioo - o 



Ammonium tartrate, . . io - o 



Ash of yeast, . . . 075 „ 



This formula has a special interest attaching to it, as it is that of 

 the first synthetically prepared medium employed in the cultivation 

 of micro-organisms. 



Colin's Fluid (as most recently ?nodified.) 



60. This consists of — 



Distilled water, . . . 200 parts. 



Tartrate of ammonium, 

 Phosphate of potassium, 

 Sulphate of magnesium, 

 Tribasic phosphate of lime, 



20 

 20 

 10 

 o - i 



Miguel's Fluid. 



61. Whilst disparaging the use of Liebig's extract of meat as a 

 nourishing medium, Miquel 1 recommends an infusion prepared 

 according to the following formula : — 



Boil 1 kilogramme of lean beef in 4 litres of distilled water, for 



five hours. 

 Strain and neutralise with caustic soda solution. Add 40 gram- 

 mes of common salt (10 gr. per litre). 

 This infusion should be sterilised by discontinuous steaming in stock- 

 flasks of convenient size, and incubated thoroughly before using it as 

 a cultivating medium. 



1 " Les organisms vivants de l'atmosphere," p. 152. 



