CONTENTS. 



CHAPTER L 

 INTRODUCTORY. 



PAGE 



General Aspects — True Position of the Subject — Relations of Micro- 

 organisms to the Tissues — Means of Ingress — Action on Tissues — 

 Acute Abscess — Tubercle — -Tissue Reaction — Digestive Ferments — 

 Specific Differences of Action — Selective Affinities — Mutability of 

 Species — Modification of Function — Requirements of Micro- 

 Organisms — Germs — Conditions necessary to Investigation, . i 



CHAPTER II. 



METHODS OF EXAMINATION. 



Chemical Reagents — -BaumgarLen's Method — Recognition of Micro- 

 organisms — Staining Reagents— Gram's Method of Staining — Stains 

 for Special Bacilli — Methods of Staining Tubercle Bacilli — Sources 

 of Error in Observation — Examination of " Fresh " Preparations — 

 His's Method of Staining and Washing — Hardening of Tissues — 

 Method of Illumination — Photography — List of Apparatus and 

 Reagents — Summary of Process of Examination of Fluids or Tissues 

 for Micro-Organisms, ...... 24 



CHAPTER III. 



SOLID CULTIVATION MEDIA. 



Nutrient Soils — Cultivation on Sterilised Potatoes— Inoculation of Potatoes 

 — Cultivation on Sterile Bread Paste — Inoculation of Bread Paste — 

 Summary of Process for preparing Sterile Bread Paste — Cultivation 

 in Sterile Nutrient Jelly — Peptonised Meat Jelly— Summary of Pro- 

 cess for preparing Sterile Nutrient Jelly — Methods of employing 

 Sterile Nutrient Jelly; in Test-Tubes; in Capsules, for testing Air 

 — Examination of Water for Micro-Organisms — Cultivation in Sterile 

 Agar-Agar Mixture — Cultivation on Sterilised Solidified Blood 

 Serum — Summary of Process for preparing Sterilised Blood Serum — 

 Method of Inoculation, ...... cc 



