STAINS FOR SPECIAL BACILLI. 



37 



Stains for Special Bacilli. 



26. Most of the known micro-organisms may be stained by the above 

 method, but there are some which require special treatment, and there 

 are probably others for which a method of staining has not yet been 

 discovered. For instance, the glanders bacillus is best stained by 

 an alkaline solution of methyl blue, which, according to Schiitz, 

 is prepared by making a one-tenth per thousand watery solution 

 of caustic potash, and adding one-third the bulk of a saturated 

 alcoholic solution of methyl blue. The sections are then treated 



fe> 



a- 



Fig. 6. — Bacilli in swollen mucous membrane of lower part of 

 small intestine. Case of typhoid fever, child. Stained by Gram's 

 method, gentian violet and eosin. ( x 300.) 



a. Epithelium of crypts. 



b. Small round cells like adenoid corpuscles, amongst which the 



violet stained bacilli (c) are found in enormous numbers. 

 Here and there are a few larger endothelioid cells. 



with dilute acetic acid, and mounted in some of the various mount- 

 ing media. 



Typhoid bacilli are also stained by Schiitz's methylene blue solu- 



