38 Instructions for the Scientific Expedition 



of the expedition. The Council also recommend that both these 

 collections be delivered up within six months after the return of the 

 expedition ; and, finally, that a report upon the botanical results of 

 the expedition be furnished to Government within six months after 

 its return, every plant or object mentioned in the report bearing 

 the number of the specimens in the collections to be delivered up 

 as above recommended. 



Zoology and Animal Physiology, 



1. Marine Invertebrata. 

 The animals which it is desirable to preserve, and which may first 

 present themselves to the notice of the naturalist in the present ex- 

 pedition, are the floating marine Mollusca and Crustacea, and those 

 which inhabit the Sargazzo or Gulph-weed. 



With respect to the Mollusca, all the species of the Cephalopoda 

 or Cuttle-fish tribe, and all the Pteropoda or lower organized floating 

 Mollusca, should be preserved. If taken alive they should be allowed 

 to die gradually in sea water, by which means they commonly remain 

 in a relaxed state, and display more of their natural outward form. 

 When dead they should be soaked for a short time in fresh water, 

 and then put into spirit; or if transparent, in the saline solution*, 

 to prevent decomposition, which otherwise rapidly takes place. 



To each specimen should be attached a number, stamped on sheet 

 tin, corresponding to the entry-number in the Catalogue, in which 

 should be noticed the kind of locomotion, or other vital phenomena, 

 and the colour of the living animal, the latter being speedily altered 

 or lost in the preserving liquor. The larger Crustacea will be liable 

 to become putrid in spirit, unless the soft mass, which fills a large 

 portion of the body, consisting of the liver, &c. be removed. Each 

 specimen of this class, excepting the very minute ones, which will 

 be best preserved in small phials or glass tubes, should be wrapt in 

 a piece of very soft, thin linen or cotton cloth, to prevent the legs 

 from being intermixed or lost, as they are very likely to fall off after 

 having been a short time in spirit. 



A very important object of investigation is the development of the 

 Crustacea, from the earliest period at which they can be observed to 

 the perfect state. They may be readily examined even before they 

 leave the egg, by opening the egg under a single microscope. 



* Common salt 1 part. 



Alum 2 parts. 



Boiling water 10 parts. 



Filter the solution when cold. 



