250 Phillips on a Comparative Study of the 



quite possible, he thought, that a dehcate connection existed 

 between them and the central body, "but," he continued, "we 

 cannot decide with certainty whether the central substance 

 of the Cyanophycese corresponds or not to the cell nucleus 

 of other organisms." 



Biitschli's investigations (7) extended over the Bacteria 

 and Cyanophycere, especially such forms as Ophidomonas 

 Jenensis, Chromatiuni Okcnvi, and other forms of bacteria 

 and spirilla from marsh water, and several forms of Bcg- 

 giatoa, Cladothrix, Oscillaria and Nostoc, all of which he 

 repeatedly investigated, and always found the cells of all 

 forms corresponding to the same general type. This type 

 consisted of a bounding membrane or cell wall, which he 

 regarded as a plasma structure. Within this was the chro- 

 matophore, a thin colored rind-layer of plasma having a 

 netted or honeycombed structure and surrounding the color- 

 less "central body." In the bacteria the central body was 

 not demonstrable in all forms, but when it was present it was 

 colorless and drawn out, and the surrounding zone was accu- 

 mulated at its poles. On staining with hsematoxylin he 

 found the "central body" to be netted, or with a coarser 

 honeycombed appearance (Wabenstruktur). At the nodal 

 points of the web-structure, in material killed in alcohol or 

 dried, he found granules which stained a red-violet with 

 weak hsematoxylin. They were very abundant and even 

 jutted out into the surrounding chromatophore and some- 

 times were found isolated in the surrounding crust layer, 

 but when so found, were always small. These he termed 

 "red granules" on account of their staining properties with 

 hjematoxylin. He could not stain them thus in material that 

 had been killed in corrosive sublimate, osmic acid mixtures, 

 or picro-sulphuric acid. They stained intensely green with 

 acetic acid methyl green. After treatment with artificial 

 gastric juice, they no longer stained characteristically with 

 the haematoxylin, but he did not think that they were digested 

 out, but had merely lost their staining properties. He denied 



