i-^i<^] Cilkeij: A Revision of fJu Tuherales of California 317 



Tuber (Eutuber) Gardnerii sp. nov. 

 Plate 30, fig. 30 



Ascocarp brown, 1.5 cm. in diam., subglobosc, with small convolu- 

 tions ; surface verrucose ; gleba white in young specimens, light brown 

 in mature plants ; veins wide, conspicuous, white ; outer cortical tissue 

 somewhat coarsely pseudopai-cnchymatous, thiii-walled, changing 

 gradually to subcortical layer of unconnected hyphae ; thickness from 

 surface to hymenium 240-320/i,; venae internae and hymenial tissue 

 of mostly slender, unconnected hyphae, 2— 4/m tliick ; venae externae of 

 loosely arranged l)rancliing hyj)hae 2-4/x thick ; asci not stipitate, semi- 

 globose, rarely elongated, 56-68 by 80-88/*; 1-5-spored ; spores light 

 brown, globose-, or long-ellipsoid, 24-30 by 28^8ft, alveolate, number 

 of alveoli varying from 3-1 1 by 5-14 across diameters ; sculpturing 

 2-4/i in height. 



"Hypogaeous under Arhntus Menziesii, Leona Heights, Alameda Co., 

 Calif., Mar. 4, 1905." "Under Qucrcus agrifoUa, U. C. Campus, 

 Berkeley, Calif., ]\Iar. 22, 1905." "Under Quercus agrifolia, U. C. 

 Campus, Berkeley, Calif., Apr. 29, 1905." 



No. 274, U. C. Col. Type. N. L. Gardner. 



This species apparently resembles most closely Tuber foetidum, T. 

 drijophilum, and T. maculatiim. However, nothing is recorded in re- 

 gard to its odor ; and its most noticeable characteristic, i.e., the exceed- 

 ing variation in number and size of alveoli upon a spore, is not men- 

 tioned in descriptions of T. foetidum, while Tulasne's figures (1851, 

 pi. XVII, fig. VII) would indicate that they are uniform, at least in 

 size. In T. foetidum the asci are described as ellipsoid and 1-2-, rarely 

 4-spored, these characters both differing from those of our specimens. 

 The species differs from the original descriptions of T. maculatum and 

 T. dryophilum and as they are described by Fischer (1897&, ])p. 47 

 and 51 respectively) in its verrucose, unspotted surface, possession of 

 pseudoparenchyinatous cortex, smaller spores, and narrower alveoli, 

 those of T. maculatum given as 7-10/x, and of T. dryophilum, 14 by 

 2\ix, though the latter is said by Fischer {ibid., p. 52) to have some- 

 times more finely alveolate spores occurring with the more common 

 widely alveolate ones. In the Californian material, the variation in 

 alveoli is very remarkable, occurring not only in a single plant, but 

 even in a single ascus. It is often the case, probably usually, that two 

 or three spores in an ascus differ widely in the size and nuinbci- of 

 alveoli. In one ascus, for example, three spores were present, the first 

 measuring 28 by 32/* and having 3 by 4 alveoli across the diameters, 

 averaging 8/i in width ; the second was 24 by 28/*, having 7 by 9 alveoli, 



