1917] Gardner: New Pacific Coast Marine Algae I 381 



The most essential difference, he says, between it and Cohn's C. 

 Lemnae is the production of two kinds of zoospores, each kind having 

 but a single cilium (concerning which he was very much in doubt), 

 and in the method of their formation, of which he says, ' ' On the cell 

 arriving at an adult stage the whole of the green protoplasmic con- 

 tents divides into a number of from ten to thirty, nearly circular, 

 zoospores, which escape through the neck-shaped portion." He was 

 not wholly satisfied that the form should be placed in the genus 

 Chlorochytrium as diagnosed by Cohn, but placed it there more or 

 less provisionally. One infers that these ''zoospores" escape singlj', 

 and not simultaneoush' within a gelatinous utricle in the manner 

 reported by later workers as occurring in the genus Chlorochytrium. 



Kjellman (1883, p. 320) described Chlorochytrium inclusum, 

 stating that it has a single chromatophore covering the entire wall. 

 He states that a large number of ''zoospores" are produced, but did 

 not state the manner of their formation, the number of their cilia, 

 nor their method of escape. De Toni (1889, vol. 1, p. 635) in diag- 

 nosing the genus Bays of the chromatophore ' ' discif orme, ' ' and of the 

 reproduction "zoospores sexualia vel neutra, " that these reproduc- 

 tive bodies have two cilia and are formed by repeated cell division. 

 Wille (1897, p. 65) diagnosed the genus as having a single parietal 

 chromatophore with manj- pj-renoids, zoospores with four cilia, and 

 gametes, both reproductive bodies formed by successive cell division, 

 and that the gametes escape in a mass surrounded by a membrane 

 in wliich they conjugate. He did not add any new features to the 

 diagnosis in the Nachtrdge which he published in 1911. The diagnosis 

 given bj' Collins (1909, p. 146) is very similar to that of Wille, but 

 he states that each kind of reproductive body has two cilia. 



The genus Chlorocystis was established by Reinhardt in 1885 

 based upon material collected near Odessa on the Black Sea, which 

 he considered to be the same as that found by Wright on the coast 

 of Ireland upon which he founded his species Chlorochytrium Cohnii. 

 Chlorocystis was published in Russian and T have not access to the 

 original paper, but I have seen and have made tracings of the draw- 

 ings. Judging from the drawings and the reviews of Reinhardt 's 

 text, as compared witli the drawings and descriptions of Wright, T 

 find myself in accord with West (1916, p. 212). It seems to me that 

 the differences between Chlorochytrium Lemnae of Cohn and the two 

 collections of plants of Wright and Reinhardt, assuming that they 

 are the same species hardly warrants the establishment of a new 



